Camelid Anti-GFP Recombinant Single Domain Antibody (clone LaG-16) (CAT#: FAMAB-0325-CN-VHH)

The antibody LaG-16 is an anti-GFP VHH sdAb which binds to GFP with high affinity. This is a high affinity sdAb directly obtained from animal serum.


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IF

Figure 1 Efficacy of LaG sdAb in immunofluorescence microscopy.

Figure 1 Efficacy of LaG sdAb in immunofluorescence microscopy.

HeLa cells transiently transfected with tubulin-emGFP marker were fixed and immunostained with LaG-16 conjugated to Alexa Fluor® 568 (AF568). Cells were visualized in the red channels, with DAPI counter-staining of nuclei (blue).

Fridy, P. C., Li, Y., Keegan, S., Thompson, M. K., Nudelman, I., Scheid, J. F., ... & Rout, M. P. (2014). A robust pipeline for rapid production of versatile sdAb repertoires. Nature methods, 11(12), 1253.

WB

Figure 2 sdAb fluorescent protein binding.

Figure 2 sdAb fluorescent protein binding.

Thirteen high-affinity LaGs were conjugated to magnetic beads and incubated with various recombinant fluorescent proteins. All LaGs bound A. victoria GFP variants.

Fridy, P. C., Li, Y., Keegan, S., Thompson, M. K., Nudelman, I., Scheid, J. F., ... & Rout, M. P. (2014). A robust pipeline for rapid production of versatile sdAb repertoires. Nature methods, 11(12), 1253.


Specifications

  • Immunogen
  • Recombinant GFP-His6
  • Host Species
  • Camelid
  • Type
  • Camelid VHH
  • Clone
  • LaG-16
  • Applications
  • WB, IF

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Western Blot and Immunofluorescence. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.

Target

  • Alternative Names
  • GFP

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Rabbit Monoclonal Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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