Human Anti-GJB2 Recombinant Antibody (clone abEC1.1) (CAT#: PABC-396)
This product is a recombinant Human antibody that can recognize Connexin26. The antibody binds an extracellular epitope of Cx26. abEC1.1 inhibits connexin hemichannels by binding an epitope at the apex of the EC1 loop.
Specific Inquiry
Inhib
Figure 1 abEC1.1 inhibits dye uptake in keratinocytes.
HaCaT cells, either wild type (T, A) or stably expressing Cx26-Venus (B-D) were bathed in extracellular medium containing: (A, B) 0 mM Ca²⁺: (C) 0 mM Ca²⁺ + 952 nM abEC1.1; (D) 2 mM Ca²⁺. Each experiment was replicated three times. Column (1) displays the cells before incubation with Lucifer Yellow (LY); column (2) shows the same field of view after the 5 min incubation with LY (1 mM, 5 min); column (3) is the difference between the two (2-1); scale bar 50 um. Box plots represent the distributions of fiuorescence values measured in 50 different cels before (1) andafter (2) incubation th LY.
Xu, L., Carrer, A., Zonta, F., Qu, Z., Ma, P., Li, S., ... & Ziraldo, G. (2017). Design and characterization of a human monoclonal antibody that modulates mutant connexin 26 hemichannels implicated in deafness and skin disorders. Frontiers in molecular neuroscience, 10, 298.
Specifications
- Immunogen
- A peptide corresponding to residues 41-56 of Cx26
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG1
- Specificity
- Human GJB2
- Species Reactivity
- Human
- Clone
- abEC1.1
- Applications
- ELISA, WB, IF, Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The use of antibody abEC1.1 in Inhibition, Enzyme-linked Immunosorbent Assay, Western Blot and Immunofluorescence has been reported. It is recommended that the optimal antibody concentration, dilution, incubation time, etc. should be carefully titrated in a specific assay.
Target
- Alternative Names
- Connexin 26; Cx26; HID; KID; PPK; DFNA3; DFNB1; NSRD1; DFNA3A; DFNB1A
- Gene ID
- 2706
- UniProt ID
- P29033
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2985z | Mouse Anti-GJB2 Recombinant Antibody (clone 24H11) | WB, ELISA, IHC | Mouse IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-396 | Human Anti-GJB2 Recombinant Antibody (clone abEC1.1); Fab Fragment | ELISA, WB, IF, Inhib | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-396 | Human Anti-GJB2 Recombinant Antibody (clone abEC1.1); scFv Fragment | ELISA, WB, IF, Inhib | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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