Rabbit Anti-GLI1 Recombinant Antibody (VS13-YC451) (CAT#: VS13-YC451)
The Rabbit Anti-GLI1 Recombinant Antibody is specific for human, mouse and rat GLI1. GLI1, a zinc finger transcription factor in sonic hedgehog signaling, regulates stem cell proliferation with gene variants modulating pathways affecting cell growth and cancer. The anti-GLI1 antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry-paraffin (1/50-1/200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65172/1154_H2_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65172/1154_H2_1_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68903/1609_F8_2_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68903/1609_F8_2_selected.jpg
Figure 3 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/991_G2_2_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/991_G2_2_selected.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/27244_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/27244_A_6_6.jpg
Figure 5 Cerebellum
(Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/27244_B_7_8.jpg
(Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/27244_B_7_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (AN3-CA, BJ hTERT+, NTERA-2, RH-30, U-266/70)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111087-GLI1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111087-GLI1
Specifications
- Immunogen
- Recombinant human Gli1, aa 38-82.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat GLI1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- GLI Family Zinc Finger 1; Glioma-Associated Oncogene Homolog 1 (Zinc Finger Protein); Oncogene GLI; GLI; Glioma-Associated Oncogene Family Zinc Finger 1; GLI-Kruppel Family Member GLI1; Glioma-Associated Oncogene 1; Glioma-Associated Oncogene; Zinc Finger Protein GLI1;
- Gene ID
- 2735
- UniProt ID
- P08151
- Long Name
- GLI Family Zinc Finger 1
- Cellular Localization
- Nucleus; Cytoplasm
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Hedgehog Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GLI1"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4082z | Mouse Anti-GLI1 Recombinant Antibody (clone 16A6) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MRO-0658-CN | Rabbit Anti-GLI1 Recombinant Antibody (clone JF09-08) | WB, IF, IHC | Rabbit IgG |
| VS3-WK93 | Mouse Anti-GLI1 Recombinant Antibody (clone F8-B9) | WB, ELISA | Mouse IgG1 |
| VS7-HM1006 | Mouse Anti-GLI1 Recombinant Antibody (clone 1B9F8) | IHC, ICC, FCM | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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