Mouse Anti-GLUL Recombinant Antibody (VS3-FY2720)
CAT#: VS3-FY2720
This anti-GLUL antibody is a mouse recombinant monoclonal antibody that is created to attach to GLUL. The calculated molecular weight of the protein is 42 kDa. It shows capability of cross-reacting with human and mouse. This monoclonal antibody is potential for conducting research on Glutamine Deficiency, Congenital and Congenital Brain Dysgenesis Due To Glutamine Synthetase Deficiency. This monoclonal antibody can be used in Western Blot, Immunohistochemistry, Immunocytochemistry, Flow cytometry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7571/10_C7_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8636/639_C3_1_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8636/22007_A_6_6.jpg
Normal Tissue
Figure 4 Soft tissue
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8636/22007_B_3_7.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7316/20133_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7316/20133_A_7_3.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, HSkMC)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135821-GLUL
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Glutamate-ammonia ligase of human and mouse origins
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC, ICC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 42 kDa.
- Related Disease
- Glutamine Deficiency, Congenital and Congenital Brain Dysgenesis Due To Glutamine Synthetase Deficiency
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Immunocytochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Glutamate-Ammonia Ligase; Glutamate Decarboxylase; Glutamine Synthetase; EC 6.3.1.2; GLNS; GS; Glutamate-Ammonia Ligase (Glutamine Synthase); Cell Proliferation-Inducing Protein 59
- Gene ID
- 2752
- Sequence Similarities
- Belongs to the glutamine synthetase family.
- Cellular Localization
- Cytoplasm, cytosol
Microsome
Mitochondrion
Cell membrane; Lipid-anchor
- Post Translation Modifications
- Palmitoylated; undergoes autopalmitoylation.
Ubiquitinated by ZNRF1.
Ubiquitination at Lys11, Lys14, Lys25, Lys91, Lys95, Lys103, Lys259, Lys268, Lys276, Lys291, Lys334, and Lys372
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001028216.1; NP_001028228.1; NP_002056.2
- Function
- May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707).
Plays a role in ribosomal 40S subunit biogenesis.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GLUL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1825z | Mouse Anti-GLUL Recombinant Antibody (clone 30C12) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MOB-0766CT | Recombinant Mouse anti-Human GLUL Monoclonal antibody (38E20) | ELISA, IP | |
| VS3-FY596 | Recombinant Rabbit Anti-GLUL Antibody (clone R03-4B6) | WB | Rabbit IgG |
| VS7-HM1010 | Mouse Anti-GLUL Recombinant Antibody (clone 2D11A2) | WB, IHC, ICC, FCM | Mouse IgG1 |
| VS7-HM1011 | Mouse Anti-GLUL Recombinant Antibody (clone 5A3B4) | WB, IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1458 | Hi-Affi™ Rabbit Anti-GLUL Recombinant Antibody (clone DS1458AB) | WB, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC420 | AbPlus™ Anti-GLUL Magnetic Beads (VS-0724-YC420) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY925 | Anti-GLUL Immunohistochemistry Kit | IHC | |
| VS-0525-XY2839 | Anti-Mouse GLUL Immunohistochemistry Kit | IHC | |
| VS-0525-XY2840 | Anti-Human GLUL Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC453 | CytoStream™ Mouse Anti-GLUL Recombinant Antibody (VS13-YC453) | WB, IHC-P, FC | Mouse IgG2a |
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