CytoStream™ Rabbit Anti-GPI Recombinant Antibody (VS13-YC461)
CAT#: VS13-YC461
The CytoStream™ Rabbit Anti-GPI Recombinant Antibody is specific for human GPI. GPI, performing glycolytic and cytokine roles, impacts energy metabolism and neural survival, with gene defects leading to disorders impacting erythrocyte metabolism and developmental processes. The anti-GPI antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/285_G6_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18655/47250_A_6_6_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/286_G6_3_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24305/284_G6_2_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40563/84798_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105220-GPI
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Specifications
- Immunogen
- Recombinant human Glucose 6 phosphate isomerase, aa 1-64.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GPI
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 10 mM PBS (pH 7.4) containing 150 mM NaCl, 50% Glycerol and 0.05% BSA.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GPI"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1303z | Mouse Anti-GPI Recombinant Antibody (clone 22C2) | ELISA, ICC, IF, IHC, WB | Mouse IgG1 |
| MOB-0757MZ | Recombinant Mouse Anti-Human GPI Antibody (clone 2C8E8) | ELISA, IHC-P, WB | Mouse antibody |
| ZG-0392J | Mouse Anti-GPI Recombinant Antibody (ZG-0392J) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-WK772 | Mouse Anti-GPI Recombinant Antibody (clone PG001) | ELISA, CLIA, RIA, IHC, WB | Mouse IgG |
| VS3-WK773 | Mouse Anti-GPI Recombinant Antibody (clone PG002) | ELISA, CLIA, RIA, IHC, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1491 | Rabbit Anti-GPI Recombinant Antibody (clone DS1491AB) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2887 | Anti-GPI Immunohistochemistry Kit | IHC | |
| VS-0525-XY2888 | Anti-Mouse GPI Immunohistochemistry Kit | IHC |
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