Rabbit Anti-GSK3B Recombinant Antibody (clone 9G11) (CAT#: ZG-0073U)
The purified Rabbit Anti-GSK3B Recombinant Antibody (clone 9G11) displayed high specificity against the GSK3B protein of human.
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WB
Figure 1 Rabbit Anti-GSK3B Antibody (ZG-0073U) in WB
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate. All lanes: GSK3 beta Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 49 kDa
Observed band size: 47 kDa
IHC
Figure 2 Rabbit Anti-GSK3B Antibody (ZG-0073U) in IHC
IHC image of ZG-0073U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-GSK3B Antibody (ZG-0073U) in IF
Immunofluorescence staining of Hela Cells with ZG-0073U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human GSK3 beta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GSK3B
- Species Reactivity
- Human
- Clone
- 9G11
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Alzheimer Disease and Bipolar Disorder
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Glycogen synthase kinase-3 beta; GSK-3 beta; EC 2.7.11.26; Serine/threonine-protein kinase GSK3B; EC 2.7.11.1; GSK3B
- Gene ID
- 2932
- UniProt ID
- P49841
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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For Research Use Only. Not For Clinical Use.
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