Mouse Anti-GST Recombinant Antibody (clone G196)
CAT#: PABC-388
This product is a recombinant Mouse antibody that can recognize GST. mAb G196 also recognized the GST fusion proteins encoded by pGEX-2T or pGEX-4T-2, in which cDNAs were inserted using BamHI and EcoRI restriction sites. The minimal epitope of the G196 mAb is the five amino acid sequence DLVPR.
Published Data
WB
Figure 1 Western blot analysis of FLAG-HA-Emerald GFP (FHG) tagged with C-terminal G196-tag in HeLa cells using mAb G196.
HeLa cells were transfected with FHG/pCDNA3 or EHG-G196/pcDNA3. After incubation for two days. the cells were lysed and subiected to Western blotting with anti-FLAG (M2) or G196 mAbs.
Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.
WB
Figure 2 Western blot analysis of Atfl-G196-GFP in fission yeast using mAb G196.
A DNA fragment containing the transcription factor atfl gene fused with the G196 epitope-GFP gene was replaced with the genomic atfl gene. Protein extracts were separated by SDS-PAGE and detected with mAb G196 and anti-GFP polyclonal antibody. GFP-Iwsl expressing cells were used as a negative control.
Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.
IP
Figure 3 Immunoprecipitation (IP) analysis of FLAG-HA-Emerald GFP (FHG) tagged with C-terminal G196-tag in HeLa cells using mAb G196.
HeLa cells were transfected with FHG/pCDNA3 or EHG-G196/pcDNA3. After incubation for two days. the cells were lysed and these lysates were immunoprecipitated with anti-FLAG (M2) (lanes 3, 4) or G196 (lanes 5, 6) mAbs and subiected to Western blotting with anti-FLAG polyclonal antibody.
Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.
IF
Figure 4 Immunofluorescence of GFP- and G196-tagged nuclear protein in fssion yeast cells using mAb G196.
Atf1-G196-GFP expressing cells were fxed and incubated with mAb G196 and anti-GFP polyclonal Ab, then stained with Alexa-594 anti-mouse and Alexa-488 anti-rabbit secondary antibodies.
Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.
IF
Figure 5 Immunofluorescence of GFP- and G196-tagged nuclear protein in HeLa cells using mAb G196.
HeLa cells were transfected with FHG-G196-NAC1/pcDNA3. Afer incubation for two days, the cells were fxed and incubated with mAb G196 and anti-GFP polyclonal Ab, then stained with Alexa-594 anti-mouse and Alexa-488 anti-rabbit secondary antibodies.
Tatsumi, K., Sakashita, G., Nariai, Y., Okazaki, K., Kato, H., Obayashi, E., ... & Urano, T. (2017). G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity. Scientific reports, 7, 43480.
❮
❯
Specifications
- Immunogen
- GST protein bacterially expressed using the pGEX-2T vector
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- GST
- Clone
- G196
- Applications
- WB, IP, IF, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The use of antibody G196 in Western Blot, Immunoprecipitation, Immunofluorescence and Enzyme-linked Immunosorbent Assay has been reported. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Western Blot: The recommended dilution for G196 is 1:2000.
Immunofluorescence: IF of GFP- and G196-tagged nuclear protein in fssion yeast cells, the dilution of mAb G196 is 1:1000.
Target
- Alternative Names
- GST; Glutathione S-transferase
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABC-388. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone G196"
- CAT
- Product Name
See other products for "GST"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0323MC | Anti-Glutathione-S-Transferase Antibody | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0119MZ | Recombinant Mouse Anti-GST (pGEX) Antibody (clone E4-64) | ELISA, RIA, WB | Mouse antibody |
| MOB-222CQ | Goat Anti-GST Recombinant Antibody (clone YC098) | ELISA, FC, IF, IM | Goat IgG |
| VS1-YJ74 | Mouse Anti-GST Recombinant Antibody (clone 2A10), HRP | WB | Mouse IgG1 |
| VS1-YJ75 | Mouse Anti-GST Recombinant Antibody (clone 2H3-D10) | WB | Mouse IgG1 |
| VS4-WK203 | Mouse Anti-GST Recombinant Antibody (VS4-WK203) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0705MZ | Chicken Anti-GST (Glutathione S-transferase) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1530 | Rabbit Anti-GST Recombinant Antibody (clone DS1530AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-388 | Mouse Anti-GST Recombinant Antibody (clone G196); Fab Fragment | ELISA, WB, IF, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-388 | Mouse Anti-GST Recombinant Antibody (clone G196); scFv Fragment | ELISA, WB, IF, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1138WJ | Recombinant Camel Anti-GST Single Domain Antibody (HPAB-1138WJ) | ELISA | Camel VHH |
| HPAB-1139WJ | Recombinant Camel Anti-GST Single Domain Antibody (HPAB-1139WJ) | ELISA | Camel VHH |
| HPAB-1140WJ | Recombinant Camel Anti-GST Single Domain Antibody (HPAB-1140WJ) | ELISA | Camel VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY93 | Mouse Anti-GST scFv-Fc Chimera (VS-0125-FY93) | WB | Mouse IgG1, scFv-Fc |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: FC, IHC, FuncS, Inhib, Cyt
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
-2.png)
Application: IB, ELISA, FC, FuncS
Application: WB, ELISA, FC, IHC, IP
Application: WB, ELISA, IF
Application: Block, IP, IF, FC
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
