Rabbit Anti-H1-2 Recombinant Antibody (clone R06-3D5)
CAT#: VS3-XY3374
This antibody is a recombinant rabbit antibody targeting the H1-2 (H1.2 linker histone, cluster member). It specifically reacts with human, mouse and rat H1-2. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat H1-2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-3D5
- Applications
- WB, IHC-P, ICC, IF, ChIP
- Related Disease
- Rumination Disorder; Narcissistic Personality Disorder
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
ChIP: 1/20
Target
- Alternative Names
- H1C; H1.2; H1F2; H1s-1; HIST1H1C
- Gene ID
- 3006
- Long Name
- H1.2 linker histone, cluster member
- Cellular Localization
- Nucleus, Chromosome
- Post Translation Modifications
- ADP-ribosylated on Ser-188 in response to DNA damage. (P16403-H12_HUMAN)
Ubiquitination at Lys17 (NX_P16403 [NX_P16403-1])
Modification sites at PhosphoSitePlus (P16403)
Glycosylation from GlyGen (P16403) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_005310.1
- Function
- Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber.
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers.
Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "H1-2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK666 | Rabbit Anti-H1-2 Recombinant Antibody (clone JU43-48) | WB, ELISA | Rabbit IgG |
| VS3-FY636 | Recombinant Rabbit Anti-HIST1H1C Antibody (clone R05-6F1) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3009 | Anti-H1-2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3010 | Anti-Mouse H1-2 Immunohistochemistry Kit | IHC |
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