Human Anti-H1N1 HA Recombinant Antibody (clone 5E4) (CAT#: HPAB-2031-FY)
This product is a recombinant human antibody that recognizes H1N1 HA. The monoclonal human antibody 5E4 reacts with the epitope of H1N1 HA.
Specific Inquiry
ELISA
Figure 1 The productivity and purification of CHO-produced antibodies against influenza virus.
ELISA of CHO-cultured medium using plates coated with influenza B antigen: B, H1N1pdm antigen: H1N1, and anti-human IgG(H þ L) antibody: Anti-Human Ab.
Misaki, R., Fukura, N., Kajiura, H., Yasugi, M., Kubota-Koketsu, R., Sasaki, T., ... & Fujiyama, K. (2016). Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes. Biologicals, 44(5), 394-402.
WB
Figure 2 CBB staining of purified antibodies produced in CHO-K1 cells and hybridomas. Arrowheads show H- and L-chains, respectively.
Misaki, R., Fukura, N., Kajiura, H., Yasugi, M., Kubota-Koketsu, R., Sasaki, T., ... & Fujiyama, K. (2016). Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes. Biologicals, 44(5), 394-402.
Neut
Figure 3 Influenza virus-neutralizing activity assay of 5A7 and 5E4.
Closed squares: CHO-produced antibodies. Open squares: Hybridoma-produced antibodies. Horizontal axis: the concentration of antibodies (mg/mL).
Misaki, R., Fukura, N., Kajiura, H., Yasugi, M., Kubota-Koketsu, R., Sasaki, T., ... & Fujiyama, K. (2016). Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes. Biologicals, 44(5), 394-402.
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- H1N1 HA
- Species Reactivity
- H1N1
- Clone
- 5E4
- Applications
- ELISA, WB, Neut
- Related Disease
- H1N1
Product Property
- Purity
- >95% as determined by SDS-PAGE analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The H1N1 HA antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Neutralization.
Target
- Alternative Names
- Influenza (H1N1) virus HA; H1N1 HA
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 5E4"
See other products for "H1N1 HA"
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-318LC | Human Anti-H1N1 HA Recombinant Antibody (MRO-318LC) | ELISA | Human IgG1, κ |
| MRO-319LC | Human Anti-H1N1 HA Recombinant Antibody (MRO-319LC) | ELISA | Human IgG1, λ |
| MRO-320LC | Human Anti-H1N1 HA Recombinant Antibody (MRO-320LC) | ELISA | Human IgG2, λ |
| MRO-321LC | Human Anti-H1N1 HA Recombinant Antibody (MRO-321LC) | ELISA | Human IgG1, λ |
| MRO-322LC | Human Anti-H1N1 HA Recombinant Antibody (MRO-322LC) | ELISA | Human IgG1, λ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4202 | Hi-Affi™ Recombinant Rabbit Anti-H1N1 HA Monoclonal Antibody (SI280DS) | WB, IP | IgG |
| MOR-4203 | Hi-Affi™ Recombinant Rabbit Anti-H1N1 HA Monoclonal Antibody (SI281DS) | WB, ELISA, IHC-P, ICC/IF, IF, FC, IP | IgG |
| MOR-4204 | Hi-Affi™ Recombinant Rabbit Anti-H1N1 HA Monoclonal Antibody (SI282DS) | WB, ELISA | IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0923LC | Recombinant Human Anti-H1N1 HA Antibody (1I20) | ELISA | IgG1 |
| EPAF-0924LC | Recombinant Human Anti-H1N1 HA Antibody (2B12) | ELISA | IgG2 |
| EPAF-1145LC | Recombinant Mouse Anti-H1N1 HA Antibody (PY102) | BL, ELISA | |
| EPAF-1146LC | Recombinant Mouse Anti-H1N1 HA Antibody (VM201) | BL, ELISA | |
| EPAF-1152LC | Recombinant Mouse Anti-H1N1 HA Antibody (IP2-11-1) | WB |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-352 | Recombinant Human Anti-H1N1 HA Antibody (CL6649) | ELISA | Human IgG |
| PABC-353 | Human Anti-H1N1 HA Recombinant Antibody (PABC-353) | ELISA, Neut | Human IgG |
| PABC-354 | Recombinant Human Anti-H1N1 HA Antibody (K1915) | ELISA, Neut | Human IgG |
| HPAB-0598-WJ | Human Anti-H1N1 HA Recombinant Antibody (HPAB-0598-WJ) | ELISA, Neut | Human IgG |
| HPAB-0599-WJ | Human Anti-H1N1 HA Recombinant Antibody (HPAB-0599-WJ) | ELISA, Neut | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-352 | Recombinant Human Anti-H1N1 HA Antibody Fab Fragment (CL6649) | ELISA | Human Fab |
| HPAB-0598-WJ-F(E) | Human Anti-H1N1 HA Recombinant Antibody; Fab Fragment (HPAB-0598-WJ-F(E)) | ELISA, Neut | Human Fab |
| HPAB-0599-WJ-F(E) | Human Anti-H1N1 HA Recombinant Antibody; Fab Fragment (HPAB-0599-WJ-F(E)) | ELISA, Neut | Human Fab |
| HPAB-0600-WJ-F(E) | Human Anti-H1N1 HA Recombinant Antibody; Fab Fragment (HPAB-0600-WJ-F(E)) | ELISA, Neut | Human Fab |
| HPAB-2031-FY-F(E) | Human Anti-H1N1 HA Recombinant Antibody (clone 5E4); Fab Fragment | IF, ELISA | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-352 | Recombinant Human Anti-H1N1 HA Antibody scFv Fragment (CL6649) | ELISA | Human scFv |
| HPAB-0598-WJ-S(P) | Human Anti-H1N1 HA Recombinant Antibody; scFv Fragment (HPAB-0598-WJ-S(P)) | ELISA, Neut | Human scFv |
| HPAB-0599-WJ-S(P) | Human Anti-H1N1 HA Recombinant Antibody; scFv Fragment (HPAB-0599-WJ-S(P)) | ELISA, Neut | Human scFv |
| HPAB-0600-WJ-S(P) | Human Anti-H1N1 HA Recombinant Antibody; scFv Fragment (HPAB-0600-WJ-S(P)) | ELISA, Neut | Human scFv |
| HPAB-2031-FY-S(P) | Human Anti-H1N1 HA Recombinant Antibody (clone 5E4); scFv Fragment | IF, ELISA | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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