Mouse Anti-H2AFX Recombinant Antibody (clone 3E1-E1-B6)
CAT#: VS3-QX496
This product is a mouse antibody that recognizes H2AFX. The antibody can be used in applications: WB, IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/769_B5_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells,non-germinal center cells and squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/166885_A_5_8_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_9_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_6_6.jpg
Normal Tissue
Figure 10 Skin
Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_1.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188486-H2AFX
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Specifications
- Immunogen
- A KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- H2AFX
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3E1-E1-B6
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:1000; IHC: 1:25
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "H2AFX"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1325 | PE-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer | FCM | |
| MHC-LC1326 | APC-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer | FCM | |
| MHC-LC1327 | BV421-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer | FCM | |
| MHC-LC2076 | PE-G*01:01/Human H2AFX (RIIPRHLQL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1553 | Rabbit Anti-H2AFX Recombinant Antibody (clone DS1553AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
| MOR-0038-FY | Rabbit Anti-H2AFX Recombinant Antibody (clone AFY0009) | ICC, IHC-P, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2333-CN | Rabbit Anti-H2AFX Recombinant Antibody (clone CBACN-624) | WB, IHC | Rabbit IgG |
| VS3-QX492 | Mouse Anti-H2AFX Recombinant Antibody (clone 2A9-B5) | WB, IF | Mouse IgG2a |
| VS3-QX493 | Mouse Anti-H2AFX Recombinant Antibody (clone 7G9-H4) | WB, IF | Mouse IgG1 |
| VS3-QX494 | Mouse Anti-H2AFX Recombinant Antibody (clone 8H4-1H11-F8) | WB, ICC | Mouse IgG1 |
| VS3-QX495 | Mouse Anti-H2AFX Recombinant Antibody (clone 3E11-1H6-G3) | WB, IF | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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