Rabbit Anti-H2AFX Recombinant Antibody (clone AFY0009)

Name: Rabbit Anti-H2AFX Recombinant Antibody (clone AFY0009)
SKU: MOR-0038-FY
Rating: 5 (1 reviews)

CAT#: MOR-0038-FY

This product is a recombinant Rabbit antibody that recognizes H2AFX. The monoclonal Rabbit antibody AFY0009 reacts with the epitope of H2AFX.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/769_B5_3_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41189/1912_D10_11_cr5c862af3f3e0f_selected.jpg

Normal Tissue Figure 3 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells,non-germinal center cells and squamous epithelial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51647/166885_A_5_8_selected.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_9_8.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_5.jpg

Normal Tissue Figure 6 Colon

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_4.jpg

Normal Tissue Figure 8 Kidney

Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_7_5.jpg

Normal Tissue Figure 9 Testis

Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_A_6_6.jpg

Normal Tissue Figure 10 Skin

Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12264/32029_B_9_1.jpg

RNA Expression Figure 11 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188486-H2AFX

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Specifications

Immunogen
Phospho-serine 139 of human Histone H2A.X.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human H2AFX

Species Reactivity
Human

Clone
AFY0009

Applications
ICC, IHC-P, WB, Inhib

Conjugate
Unconjugated

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Format
Lyophilized

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS, 5% Trehalose

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot, Inhibition.

Target

Alternative Names
H2A Histone Family Member X; Histone H2A.X; H2A/X; H2AX; H2A Histone Family, Member X; H2AX Histone; Histone H2AX; H2A.X

Gene ID
3014

UniProt ID
P16104

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOR-0038-FY.pdf

MSDS

MSDS - MOR-0038-FY.pdf

COA

Certificate of Analysis Lookup

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See other products for "H2AFX"

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CAT	Product Name	Application	Type
MOB-2435MZ	Recombinant Mouse Anti-Human H2AFX Antibody (clone 4G3)	ELISA, FC, ICC, IF, IHC-P, WB	Mouse antibody
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VS3-QX492	Mouse Anti-H2AFX Recombinant Antibody (clone 2A9-B5)	WB, IF	Mouse IgG2a
VS3-QX493	Mouse Anti-H2AFX Recombinant Antibody (clone 7G9-H4)	WB, IF	Mouse IgG1
VS3-QX494	Mouse Anti-H2AFX Recombinant Antibody (clone 8H4-1H11-F8)	WB, ICC	Mouse IgG1

CAT	Product Name	Application
MHC-LC1325	PE-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer	FCM
MHC-LC1326	APC-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer	FCM
MHC-LC1327	BV421-HLA-G/Human H2AFX (RIIPRHLQL) MHC Tetramer	FCM
MHC-LC2076	PE-G*01:01/Human H2AFX (RIIPRHLQL) MHC Tetramer	FCM

CAT	Product Name	Application	Type
MOR-1553	Rabbit Anti-H2AFX Recombinant Antibody (clone DS1553AB)	ICC, IF, IHC-P, WB	Rabbit IgG

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