Rabbit Anti-H2BC12 Recombinant Antibody (clone 4G10) (CAT#: ZG-0719J)
This product is a rabbit antibody that recognizes H2BC12.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-H2BC12 Antibody (ZG-0719J) in WB
Western Blot
Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue
All lanes: Histone H2B type 1-K antibody at 0.16μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 KDa
Observed band size: 14 KDa
IHC
Figure 2 Rabbit Anti-H2BC12 Antibody (ZG-0719J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human H2BC12
- Species Reactivity
- Human
- Clone
- 4G10
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Infections
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:5000-1:10000, IHC:1:50-1:500
Target
- Alternative Names
- H2BK; H2B/S; H2BFT; H2BFAiii; HIST1H2BK; histone H2B type 1-K; H2B K; H2B histone family, member T; HIRA-interacting protein 1; histone 1, H2bk; histone cluster 1 H2B family member k; histone cluster 1, H2bk; histone family member
- Gene ID
- 85236
- UniProt ID
- O60814
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY642 | Recombinant Rabbit Anti-HIST1H2BK Antibody (clone R05-1J4) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY643 | Recombinant Rabbit Anti-HIST1H2BK Antibody (clone R07-8K4) | WB, IHC-P, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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