Mouse Anti-H3C1 Recombinant Antibody (clone 1G1)
CAT#: VS3-WK1570
This antibody is a mouse recombinant antibody against human H3C1, which is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. The clone 1G1 can be used in WB analysis at a dilution of 1/2000-5000, IF analysis at a dilution of 1/100-500, IP analysis at a dilution of 1/200, and IHC analysis at a dilution of 1/50-300.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000275714-H3C1
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Specifications
- Immunogen
- Recombinant Protein of Histone H3
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human H3C1
- Species Reactivity
- Human, Mouse, Rat, Yeast
- Clone
- 1G1
- Applications
- WB, IHC-P, IF, ICC, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4, containing 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/2000-5000
IF: 1/100-500
IP: 1:200
IHC: 1/50-1/300
Target
- Alternative Names
- H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A
- Gene ID
- 8350
- UniProt ID
- P68431
- Long Name
- H3 clustered histone 1
- Sequence Similarities
- Belongs to the histone H3 family.
- Cellular Localization
- Chromosome, Nucleosome core, Nucleus
- Post Translation Modifications
- Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
- Protein Refseq
- NP_003520.1
- Function
- Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "Clone 1G1"
- CAT
- Product Name
See other products for "H3C1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0720J | Rabbit Anti-H3C1 Recombinant Antibody (clone 6F6) | ELISA, WB, ICC, IF, FC | Rabbit IgG |
| ZG-0721J | Rabbit Anti-H3C1 Recombinant Antibody (clone 3E10) | ELISA, WB, ICC, IF | Rabbit IgG |
| ZG-0722J | Rabbit Anti-H3C1 Recombinant Antibody (clone 3H3) | ELISA, WB, ICC, IF | Rabbit IgG |
| ZG-0723J | Rabbit Anti-H3C1 Recombinant Antibody (clone 2A5) | ELISA, WB, ICC, IF | Rabbit IgG |
| ZG-0724J | Rabbit Anti-H3C1 Recombinant Antibody (clone 1B2) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3031 | Anti-H3C1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3032 | Anti-Mouse H3C1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3033 | Anti-Rat H3C1 Immunohistochemistry Kit | IHC |
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