Rabbit Anti-H3C1 Recombinant Antibody (clone 2B5) (CAT#: ZG-0729J)
This product is a rabbit antibody that recognizes H3C1.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-H3C1 Antibody (ZG-0729J) in WB
Western Blot
Positive WB detected in MCF-7 whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysate,SH-SY5Y whole cell lysate,Rat brain tissue
All lanes Mono-methyl-Histone H3.1(K18)antibody at 0.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa
ICC
Figure 2 Rabbit Anti-H3C1 Antibody (ZG-0729J) in ICC
Immunocytochemistry analysis of this product diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-H3C1 Antibody (ZG-0729J) in IF
Immunofluorescence staining of Hela cells with this product at 1:43,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human H3C1
- Species Reactivity
- Human
- Clone
- 2B5
- Applications
- ELISA, WB, ICC, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Immunofluorescence.
WB:1:500-1:2000, ICC:1:50-1:500, IF:1:30-1:200
Target
- Alternative Names
- H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; histone H3.1; H3 histone family, member A; histone 1, H3a; histone H3/a; histone cluster 1 H3 family member a; histone cluster 1, H3a
- Gene ID
- 8350
- UniProt ID
- P68431
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "H3C1"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK2176 | Rabbit Anti-H3C1 (pS10) Recombinant Antibody (clone BP6092) | IHC-P | Rabbit IgG |
| VS3-WK2196 | Rabbit Anti-H3C1 (acetyl K14) Recombinant Antibody (clone R06-2F5) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-WK2197 | Rabbit Anti-H3C1 (acetyl K27) Recombinant Antibody (clone R49-6E-4) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK2199 | Rabbit Anti-H3C1 (acetyl K56) Recombinant Antibody (clone R02-5J8) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK2207 | Rabbit Anti-H3C1 (monomethyl R17) Recombinant Antibody (clone R01-7M5) | WB, IHC-P, ICC, IF | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0729J. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: ELISA, IP, FC, FuncS, Neut, IF, WB
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: FC, IHC, FuncS, Inhib, Cyt
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: FC, IHC, Cyt, FuncS
Application: ELISA, FC, Neut, Inhib
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
