Mouse Anti-H3C1 (trimethyl K79) Recombinant Antibody (VS3-WK2216)
CAT#: VS3-WK2216
This product is a mouse recombinant antibody that is specific to methylated H3C1. The target of this antibody is Tri Methyl-H3C1 (Lys79). It can be used in WB analysis at a dilution of 1/500-1/2000, IF analysis at a dilution of 1/200, IP analysis at a dilution of 1/200, and IHC staining at a dilution of 1/50-1/300.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000275714-H3C1
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Specifications
- Immunogen
- Synthetic peptide of Histone H3 (Tri-methyl Lys79)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Trimethyl-Lys79 of human, mouse and rat H3C1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/2000
IP: 1/200
IF: 1/200
IHC: 1/50-1/300
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "H3C1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK2176 | Rabbit Anti-H3C1 (pS10) Recombinant Antibody (clone BP6092) | IHC-P | Rabbit IgG |
| VS3-WK2196 | Rabbit Anti-H3C1 (acetyl K14) Recombinant Antibody (clone R06-2F5) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-WK2197 | Rabbit Anti-H3C1 (acetyl K27) Recombinant Antibody (clone R49-6E-4) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK2199 | Rabbit Anti-H3C1 (acetyl K56) Recombinant Antibody (clone R02-5J8) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK2207 | Rabbit Anti-H3C1 (monomethyl R17) Recombinant Antibody (clone R01-7M5) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3031 | Anti-H3C1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3032 | Anti-Mouse H3C1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3033 | Anti-Rat H3C1 Immunohistochemistry Kit | IHC |
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