F045-092 was extensively analyzed. It neutralized not only H3N2 but also H1N1, H2N2 and H5N1 viruses, although the activities were largely varied. Flow cytometry suggested that it have the ability to bind to HA and HA1 artificially expressed on the cell surface. It is likely that F045-092 bind to a conserved epitope in the head region formed by HA1. Interestingly, while the VH1-69 gene can encode mAbs against the HA stem that are group 1 specific, F045-092 and its relatives that recognize the head region also use VH1-69.
Figure 1 F045-092 and F026-427 binding to virus strains.
Western blot of three H3 strain viruses and one H1 strain virus with Fab-cp3 of four clones under nonreducing conditions.
Figure 2 Binding activities of IgG from F045-092 (gray) and F026-427 (white) to 12 H3 viruses and 1 H1 virus were measured by ELISA.
The OD values at 492 nm are indicated.
Figure 3 FCM analyses of the cells expressing HA and HA1 of Aichi/68 virus.
FCM signals for mock transfection (gray-filled area), HA-expressing cells (green), and HA1-expressing cells (pink) are shown.
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