Mouse Anti-HA-Tag Recombinant Antibody (clone 18B11H6) (CAT#: ZG-0285U)

Figure 1 Mouse Anti-HA-Tag Antibody (ZG-0285U) in WB

Positive WB detected in: 3 different overexpression lysates with HA tagged All lanes: HA-Tag antibody at 1: 1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 35, 48 kDa
Observed band size: 35, 35, 48 kDa

Figure 2 Mouse Anti-HA-Tag Antibody (ZG-0285U) in IF

Immunofluorescence staining of 293F transfected cells with ZG-0285U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 3 Mouse Anti-HA-Tag Antibody (ZG-0285U) in IP

Immunoprecipitating HA-Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG (1µg) in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0285U (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg)

Figure 4 Mouse Anti-HA-Tag Antibody (ZG-0285U) in FC

Overlay histogram showing 293F transfected cells stained with ZG-0285U (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.