Rabbit Anti-MTOR Polyclonal Antibody (CAT#: VS3-WK1564)

• Immunogen
• Synthesized peptide derived from human mTOR

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human MTOR

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Clonality
• Polyclonal

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• PBS, 0.5% BSA, 50% Glycerol

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:500) and ELISA (1:10000-1/20000).

• Alternative Names
• Mechanistic Target Of Rapamycin Kinase; Rapamycin And FKBP12 Target 1; Mammalian Target Of Rapamycin; FK506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; Mechanistic Target Of Rapamycin (Serine/Threonine Kinase); FK506 Binding Protein 12-Rapamycin Associated Protein 2; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin Associated Protein FRAP2; FKBP-Rapamycin Associated Protein; Mechanistic Target Of Rapamycin; Rapamycin Target Protein 1; FRAP1; FRAP2

• Gene ID
• 2475

• UniProt ID
• P42345

• Long Name
• Mechanistic Target Of Rapamycin Kinase

• Sequence Similarities
• Belongs to the PI3/PI4-kinase family.

• Cellular Localization
• Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Lysosome, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Nucleus

• Post Translation Modifications
• Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity.

• Protein Refseq
• NP_004949.1

• Function
• Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E) (By similarity).

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1517-YJ
• Sheep Anti-Rabbit IgG-heavy and light chain Secondary antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody