Recombinant Mouse Anti-HBV Antibody (E183/A2) (CAT#: HPAB-1539-FY)

This product is a recombinant antibody that recognizes HBV. The monoclonal antibody E183/A2 reacts with the epitope of HBV.

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IF

Figure 1 Recognition of pMHC complexes on HBV-infected cells.

Visualization of the pMHC complex. HBV-producing HepG2-117 cells or control HepG2 cells were fixed with 1% PFA and stained with 1 ug of Env183/A2 MAb or a negative control EBNA/A2 MAb overnight at 4°C.

Sastry, K. S., Too, C. T., Kaur, K., Gehring, A. J., Low, L., Javiad, A., ... & Macary, P. A. (2011). Targeting hepatitis B virus-infected cells with a T-cell receptor-like antibody. Journal of virology, 85(5), 1935-1942.

FC

Figure 2 Schematic representation of the production of Env183/A2 MAb.

Histograms representing the different staining profiles of B-cell hybridoma supernatants. T2 cells were pulsed with 1 M (each) Env183-91 peptide or influenza A virus matrix peptide at positions 58 to 66 (Flu M158-66) and then incubated with hybridoma supernatant followed by washing and incubation with anti-mouse IgG-Alexa Fluor 488 for 30 min.

Sastry, K. S., Too, C. T., Kaur, K., Gehring, A. J., Low, L., Javiad, A., ... & Macary, P. A. (2011). Targeting hepatitis B virus-infected cells with a T-cell receptor-like antibody. Journal of virology, 85(5), 1935-1942.

FC

Figure 3 Fine-mapping of Env183/A2 MAb epitope recognition.

Env183/A2 MAb is exclusively specific for the Env183-91 peptide of HBV genotypes (gen) A, C, and D. T2 cells were pulsed with 1 M Env183-91 peptides with the indicated substitutions at position 187.

Sastry, K. S., Too, C. T., Kaur, K., Gehring, A. J., Low, L., Javiad, A., ... & Macary, P. A. (2011). Targeting hepatitis B virus-infected cells with a T-cell receptor-like antibody. Journal of virology, 85(5), 1935-1942.

IF

Figure 4 Targeted delivery of immunofluorochrome.

At different time points after incubation, coverslips were collected and incubated with DiI red to label the plasma membrane (red) or LysoTracker red dye to trace lysosomes in the cells (red, bottom row). Cell nuclei were stained with DAPI (blue). Cells on coverslips were fixed with 1% PFA and mounted for confocal imaging. Cells pulsed with an irrelevant M1 peptide were used as negative controls. Data represent those typical of two independent experiments.

Sastry, K. S., Too, C. T., Kaur, K., Gehring, A. J., Low, L., Javiad, A., ... & Macary, P. A. (2011). Targeting hepatitis B virus-infected cells with a T-cell receptor-like antibody. Journal of virology, 85(5), 1935-1942.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • HBV
  • Species Reactivity
  • HBV
  • Clone
  • E183/A2
  • Applications
  • IF, FC

Applications

  • Application Notes
  • The HBV antibody has been reported in applications of Immunofluorescence, Flow Cytometry.
    FC: Ab or mouse isotype control Ab for 45 min at 4°C. After washing, anti-mouse IgG-allophycocyanin (APC) secondary antibody was added at 4°C for 20 min. The cells were then fixed and permeabilized by incubation in fixation and permeabilization buffer for 30 min at 4°C and then stained with rabbit anti-human albumin antibody or rabbit isotype (1 ug/ml) for 45 min, washed with permeabilization buffer, and incubated with anti-rabbit IgG-phycoerythrin (PE) secondary antibodies (20 min at 4°C). After a further washing step, cells were analyzed with a BD FACSCanto flow cytometer.

Target

  • Alternative Names
  • Hepatitis B virus preS1; HBV

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "HBV"


For research use only. Not intended for any clinical use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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