Recombinant Human Anti-HBV Antibody (C9G9)

Figure 1 HBV-neutralizing activity of the bispecific antibodies.

(D) The binding of C1G1 and 2B5F5 to HBsAg was markedly reduced when HBsAg was preincubated with C4D2-BsAb but not with C4G4 plus D2H2. C4G4 plus D2H2, or C4D2-BsAb was first incubated with biotinHBsAg. The mixtures were then transferred to the plates coated with anti-HBsAg antibodies A3E3, A6E6, B7F7, C1G1, C9G9, D10H10, 2B5F5, 2C7G7, 2D4H4 and 2D5H5. After 2 h incubation, the plates were washed and biotin-HBsAg bound were detected with HRP-strepavidin conjugated. Results were shown as mean ± SD of three independent experiments.

Tan, W., Meng, Y., Li, H., Chen, Y., Han, S., Zeng, J., ... & Guo, Y. (2013, November). A bispecific antibody against two different epitopes on hepatitis B surface antigen has potent hepatitis B virus neutralizing activity. In MAbs (Vol. 5, No. 6, pp. 946-955). Taylor & Francis.