Recombinant Llama Anti-HCEC Single Domain Antibody (FC44) (CAT#: HPAB-1263WJ)

Figure 1 A graph shows the binding of sdAb fusion proteins to endothelial cells.

Binding of FC44 (black bars), FC5 (grey bars), and NC11 (open bars)-c-myc-His5 fusion proteins to cell lysates of human cerebromicrovascular endothelial cells (HCEC), human pial artery endothelial cells (HPEC), human umbilical vein endothelial cells (HUVEC), human lung microvascular endothelial cells (HLMEC), and fetal human astrocytes (FHAs).

Figure 2 Depicts the binding of sdAb fusion proteins to endothelial cells.

Binding of FC44 (black bars), FC5 (grey bars), and NC11 (open bars)-c-myc-His5 fusion proteins to live HCEC and HLMEC.

Figure 3 A photograph shows the affinity purification of the antigen/sdAb complex from HCEC.

A gradient elution of the antigen/antibody complex with increasing strengths of imidazole buffer. Denatured FC44/antigen complex was resolved on 12% SDS-PAGE, transferred to nitrocellulose membrane, and antigen was detected by probing the membrane with FC44 fusion protein and subsequently with anti-c-myc antibody.

Figure 4 Shows the affinity purification of the antigen-sdAb complex.

Silver stained gel of respective resolved FC44, FC5, and NC11 antigens eluted from the Ni2⁺ affinity column with 400 mM imidazole.

Figure 5 The uptake of FC44 into living HCEC.

Alexafluor 488/532-labeled sdAbs were added to cells for 3 h, and the uptake was determined by fluorescence microscopy

Muruganandam, A., Tanha, J., Narang, S., & Stanimirovic, D. (2002). Selection of phage-displayed llama single-domain antibodies that transmigrate across human blood–brain barrier endothelium. The FASEB Journal, 16(2), 240-242.