Mouse Anti-HCK Recombinant Antibody (VS3-FY2732)
CAT#: VS3-FY2732
This anti-HCK antibody is a mouse recombinant monoclonal antibody that is specifically created to bind HCK. The calculated molecular weight of the antigen is 59.6 kDa. It exhibits cross-reactivity with human, mouse and rat. The antibody can be utilized for detection of HCK by Western Blot, Immunocytochemistry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35534/2069_B3_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63768/2005_C2_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows cytoplasmic positivity in syncytiotrophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/13714_B_7_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/13714_A_8_4.jpg
Normal Tissue
Figure 6 Testis
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/13714_A_6_6.jpg
Normal Tissue
Figure 7 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5195/13714_B_6_4.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (THP-1, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101336-HCK
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- HCK proto-oncogene, Src family tyrosine kinase of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 59.6 kDa.
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunocytochemistry: 1/10-1/25
Target
- Alternative Names
- HCK Proto-Oncogene, Src Family Tyrosine Kinase; Hemopoietic Cell Kinase; Hematopoietic Cell Kinase; P59-HCK/P60-HCK; EC 2.7.10.2; P59Hck
- Gene ID
- 3055
- UniProt ID
- P08631
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Lysosome, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases.
Ubiquitinated by CBL, leading to its degradation via the proteasome.
Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.
- Protein Refseq
- NP_001165600.1; NP_001165601.1; NP_001165602.1
- Function
- Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Secondary Antibody
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- Product Name
See other products for "HCK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3439z | Mouse Anti-HCK Recombinant Antibody (clone 40H4) | WB, IP, IF | Mouse IgG1 |
| MOB-1865MZ | Recombinant Mouse Anti-Human HCK Antibody (clone 4E23F20) | ELISA, IHC-P, WB | Mouse antibody |
| VS3-CJ511 | Mouse Anti-HCK Recombinant Antibody (VS3-CJ511) | WB, ICC | Mouse IgG1, κ |
| VS3-XY798 | Mouse Anti-HCK Recombinant Antibody (clone 3D12E10) | ELISA, WB, IHC | Mouse IgG1 |
| VS7-0425-WR18 | Mouse Anti-HCK Monoclonal Antibody (VS7-0425-WR18) | WB, IHC-P, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1570 | Rabbit Anti-HCK Recombinant Antibody (clone DS1570AB) | FC, ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3080 | Anti-HCK Immunohistochemistry Kit | IHC |
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