Human Anti-HCMV gB Recombinant Antibody (clone 1G2) (CAT#: FAMAB-0143JF)
This is a human IgG3-lambda antibody specially binds to domain I of glycoprotein B (gB) of human cytomegalovirus (HCMV). Clone 1G2 has a reactivity with domain I peptide (aa 132-344) and has a 50% neutralization at concentrations of 0.2-1.3 mg/ml.
Specific Inquiry
IHC
Figure 1 EM two-dimensional class averages of gB-698glyco (top panels) and gB-698glyco in complex with 1G2 Fab.
Each value represents the mean ± S.E.M. of duplicate measurements from at least four independent experiments.
Chandramouli, S., Ciferri, C., Nikitin, P. A., Caló, S., Gerrein, R., Balabanis, K., ... & Carfi, A. (2015). Structure of HCMV glycoprotein B in the postfusion conformation bound to a neutralizing human antibody. Nature communications, 6(1), 1-12.
FC
Figure 2 FACS analysis of BHK cells electroporated with RNA encoding full-length gB and stained with monoclonal antibodies 1G2 and ITC88 24 h post electroporation.
The gB staining histograms were gated on viable, dsRNA+ cells.
Chandramouli, S., Ciferri, C., Nikitin, P. A., Caló, S., Gerrein, R., Balabanis, K., ... & Carfi, A. (2015). Structure of HCMV glycoprotein B in the postfusion conformation bound to a neutralizing human antibody. Nature communications, 6(1), 1-12.
WB
Figure 3 Western blot analysis of purified VR1814 virions immunoprecipitated with 1G2 or ITC88.
Samples were resolved on SDS–polyacrylamide gel electrophoresis and detected with ITC88 mAb.
Chandramouli, S., Ciferri, C., Nikitin, P. A., Caló, S., Gerrein, R., Balabanis, K., ... & Carfi, A. (2015). Structure of HCMV glycoprotein B in the postfusion conformation bound to a neutralizing human antibody. Nature communications, 6(1), 1-12.
Specifications
- Host Species
- Human
- Type
- Human IgG3, λ
- Specificity
- HCMV gB
- Species Reactivity
- HCMV
- Clone
- 1G2
- Applications
- WB, FC, IHC
- Related Disease
- HCMV infection
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HCMV gB antibody has been reported in applications of Western Blot, Flow Cytometry, Immunohistochemistry.
Target
- Alternative Names
- Human Cytomegalovirus; HCMV; Human Cytomegalovirus envelope glycoprotein B; HCMV gB
- Gene ID
- 3077424
- UniProt ID
- F5HB53
- Function
- Neutralization
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-443 | Recombinant Human Anti-HCMV GB Antibody | FC, Neut, Funcs | IgG |
| HPAB-0639-CN | Recombinant Human Anti-HCMV gB Antibody (2F10) | ELISA, Neut | Human IgG |
| HPAB-0640-CN | Recombinant Human Anti-HCMV gB Antibody (2M16) | ELISA, Neut | Human IgG |
| HPAB-0641-CN | Recombinant Human Anti-HCMV gB Antibody (2N9) | ELISA, Neut | Human IgG |
| FAMAB-0582WJ | Human Anti-HCMV gB Recombinant Antibody (clone h272.7) | ELISA, WB, Neut | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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