Human Anti-HCMV GB Recombinant Antibody (clone 5A6) (CAT#: FAMAB-1777CQ)

Figure 1 U373 cells expressing gH or gB were subjected to immunoprecipitation studies.

Total cell lysates from U373gB (lanes 1-8) and U373gH (lanes 12-19) were exposed to the CMV-neutralizing mAbs. The recovered immune complexes were resolved by SDS-PAGE and exposed to anti-gB (lanes 1-8) or anti-gH (lanes 12-19) antibody. Total cell lysates from U373gB (lanes 10-11) and U373gH (lanes 20-21) validated expression of the respective CMV protein in each cell type. Relative molecular mass markers are indicated in all relevant figures.

Gardner, T. J., Stein, K. R., Duty, J. A., Schwarz, T. M., Noriega, V. M., Kraus, T., ... & Tortorella, D. (2016). Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein. Nature communications, 7(1), 1-15.

Figure 2 U373 cells expressing gH or gB were subjected to flow cytometry.

U373gH (white peaks) and U373gB (grey peaks) cells were permeabilized and exposed to the neutralizing CMV mAbs followed by detection with flow cytometry. mAb 14-4b was used as a positive control for labelling of the gH protein.

Gardner, T. J., Stein, K. R., Duty, J. A., Schwarz, T. M., Noriega, V. M., Kraus, T., ... & Tortorella, D. (2016). Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein. Nature communications, 7(1), 1-15.

Figure 3 Recognition of CMV gB by the α-gB mAbs as demonstrated by immunoprecipitation studies from TB40/E WT-infected cells.

MRC5 cells infected with TB40/E were harvested at 96 hpi and total cell lysates were exposed to the anti-gB antibodies. Recovered immune complexes were resolved by SDS-PAGE and exposed to anti-gB immunoblot (lanes 1-5). Relative molecular mass markers are indicated.

Gardner, T. J., Stein, K. R., Duty, J. A., Schwarz, T. M., Noriega, V. M., Kraus, T., ... & Tortorella, D. (2016). Functional screening for anti-CMV biologics identifies a broadly neutralizing epitope of an essential envelope protein. Nature communications, 7(1), 1-15.