This product is a humanized monoclonal antibody that is specific for HCMV gB. This antibody can be used in a variety of applications, such as ELISA, WB, Neut.
Figure 1 Complement-dependent neutralization by selected gB mAbs in MRC-5 cells.
Antibody r272.7 and its humanized version h272.7, along with r350.1 and CytoGam®, were tested for neutralization with no complement, or with rabbit complement or human complement in MRC-5 cells. IC50 values, defined as IgG concentrations required to neutralize 50% of viral infectivity were calculated by four-parameter curve fitting. Rabbit complement was added at 1:64 final dilution and human complement at 1:32. Human complement was sourced from a healthy donor of HCMV seronegative status.
Li, F., Freed, D. C., Tang, A., Rustandi, R. R., Troutman, M. C., Espeseth, A. S., ... & Fu, T. M. (2017). Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination. NPJ vaccines, 2(1), 1-8.
Figure 2 Complement-dependent neutralization by selected gB mAbs in ARPE-19 cells.
Antibody r272.7 and its humanized version h272.7, along with r350.1 and CytoGam®, were tested for neutralization with no complement, or with rabbit complement or human complement in ARPE-19 cells. IC50 values, defined as IgG concentrations required to neutralize 50% of viral infectivity were calculated by four-parameter curve fitting. Rabbit complement was added at 1:64 final dilution and human complement at 1:32. Human complement was sourced from a healthy donor of HCMV seronegative status.
Li, F., Freed, D. C., Tang, A., Rustandi, R. R., Troutman, M. C., Espeseth, A. S., ... & Fu, T. M. (2017). Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination. NPJ vaccines, 2(1), 1-8.
Figure 3 Western blot analysis
Biochemical property of r272.7 is different from that of r350.1 and r240.4. HCMV virus (AD169rev-GFP) was denatured and reduced for antibody detection in Western blot analysis.
Li, F., Freed, D. C., Tang, A., Rustandi, R. R., Troutman, M. C., Espeseth, A. S., ... & Fu, T. M. (2017). Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination. NPJ vaccines, 2(1), 1-8.
Figure 4 ELISA analysis
Three rabbit antibodies in titration were tested to compete with h272.7 at a fixed concentration in ELISA.
Li, F., Freed, D. C., Tang, A., Rustandi, R. R., Troutman, M. C., Espeseth, A. S., ... & Fu, T. M. (2017). Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination. NPJ vaccines, 2(1), 1-8.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-443-F(E) | Recombinant Human Anti-HCMV GB Antibody Fab Fragment | WB, Neut, FuncS | Fab |
FAMAB-0142JF-F(E) | Human Anti-HCMV gB Recombinant Antibody (clone SM10); Fab Fragment | WB, ELISA, FuncS | Human Fab |
FAMAB-0143JF-F(E) | Human Anti-HCMV gB Recombinant Antibody (clone 1G2); Fab Fragment | WB, ELISA, FuncS | Human Fab |
FAMAB-0144JF-F(E) | Human Anti-HCMV gB Recombinant Antibody (clone 2C2); Fab Fragment | WB, ELISA, FuncS | Human Fab |
FAMAB-0145JF-F(E) | Human Anti-HCMV gB Recombinant Antibody (clone SM1-6); Fab Fragment | WB, ELISA, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-1032LC | Anti-HCMV gB Recombinant Antibody (TI-23) | ELISA | Humanized antibody |
MRO-1032LC-S(P) | Anti-HCMV gB Recombinant Antibody scFv Fragment (TI-23) | ELISA | Humanized antibody |
MRO-1032LC-F(E) | Anti-HCMV gB Recombinant Antibody Fab Fragment (TI-23) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-1042LC-F(E) | Anti-HCMV gB Recombinant Antibody Fab Fragment (4H9) | ELISA | Human antibody |
MRO-1043LC-F(E) | Anti-HCMV gB Recombinant Antibody Fab Fragment (5F1) | ELISA | Human antibody |
MRO-1044LC-F(E) | Anti-HCMV gB Recombinant Antibody Fab Fragment (10C6) | ELISA | Human antibody |
MRO-1048LC-F(E) | Anti-HCMV gB Recombinant Antibody Fab Fragment (ITC33) | ELISA | Human antibody |
MRO-1049LC-F(E) | Anti-HCMV gB Recombinant Antibody Fab Fragment (ITC39) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-1438LC | Recombinant Human Anti-HCMV gB Antibody (MAb C23) | WB | IgG1 |
EPAF-1497LC | Recombinant Mouse Anti-HCMV gB Antibody (CH28-2) | ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0639-CN | Recombinant Human Anti-HCMV gB Antibody (2F10) | ELISA, Neut | Human IgG |
HPAB-0640-CN | Recombinant Human Anti-HCMV gB Antibody (2M16) | ELISA, Neut | Human IgG |
HPAB-0641-CN | Recombinant Human Anti-HCMV gB Antibody (2N9) | ELISA, Neut | Human IgG |
HPAB-0642-CN | Recombinant Human Anti-HCMV gB Antibody (4P12) | ELISA, Neut | Human IgG |
HPAB-0643-CN | Recombinant Human Anti-HCMV gB Antibody (5P9) | ELISA, Neut | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0639-CN-S(P) | Recombinant Human Anti-HCMV gB Antibody scFv Fragment (2F10) | ELISA, Neut | Human scFv |
HPAB-0640-CN-S(P) | Recombinant Human Anti-HCMV gB Antibody scFv Fragment (2M16) | ELISA, Neut | Human scFv |
HPAB-0641-CN-S(P) | Recombinant Human Anti-HCMV gB Antibody scFv Fragment (2N9) | ELISA, Neut | Human scFv |
HPAB-0642-CN-S(P) | Recombinant Human Anti-HCMV gB Antibody scFv Fragment (4P12) | ELISA, Neut | Human scFv |
HPAB-0643-CN-S(P) | Recombinant Human Anti-HCMV gB Antibody scFv Fragment (5P9) | ELISA, Neut | Human scFv |
There are currently no Customer reviews or questions for FAMAB-0582WJ. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.