Human Anti-HCMV gB Recombinant Antibody (clone h272.7) (CAT#: FAMAB-0582WJ)
This product is a humanized monoclonal antibody that is specific for HCMV gB. This antibody can be used in a variety of applications, such as ELISA, WB, Neut.
Specific Inquiry
Neut
Figure 1 Complement-dependent neutralization by selected gB mAbs in MRC-5 cells.
Antibody r272.7 and its humanized version h272.7, along with r350.1 and CytoGam®, were tested for neutralization with no complement, or with rabbit complement or human complement in MRC-5 cells. IC50 values, defined as IgG concentrations required to neutralize 50% of viral infectivity were calculated by four-parameter curve fitting. Rabbit complement was added at 1:64 final dilution and human complement at 1:32. Human complement was sourced from a healthy donor of HCMV seronegative status.
Li, F., Freed, D. C., Tang, A., Rustandi, R. R., Troutman, M. C., Espeseth, A. S., ... & Fu, T. M. (2017). Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination. NPJ vaccines, 2(1), 1-8.
Neut
Figure 2 Complement-dependent neutralization by selected gB mAbs in ARPE-19 cells.
Antibody r272.7 and its humanized version h272.7, along with r350.1 and CytoGam®, were tested for neutralization with no complement, or with rabbit complement or human complement in ARPE-19 cells. IC50 values, defined as IgG concentrations required to neutralize 50% of viral infectivity were calculated by four-parameter curve fitting. Rabbit complement was added at 1:64 final dilution and human complement at 1:32. Human complement was sourced from a healthy donor of HCMV seronegative status.
Li, F., Freed, D. C., Tang, A., Rustandi, R. R., Troutman, M. C., Espeseth, A. S., ... & Fu, T. M. (2017). Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination. NPJ vaccines, 2(1), 1-8.
WB
Figure 3 Western blot analysis
Biochemical property of r272.7 is different from that of r350.1 and r240.4. HCMV virus (AD169rev-GFP) was denatured and reduced for antibody detection in Western blot analysis.
Li, F., Freed, D. C., Tang, A., Rustandi, R. R., Troutman, M. C., Espeseth, A. S., ... & Fu, T. M. (2017). Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination. NPJ vaccines, 2(1), 1-8.
ELISA
Figure 4 ELISA analysis
Three rabbit antibodies in titration were tested to compete with h272.7 at a fixed concentration in ELISA.
Li, F., Freed, D. C., Tang, A., Rustandi, R. R., Troutman, M. C., Espeseth, A. S., ... & Fu, T. M. (2017). Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination. NPJ vaccines, 2(1), 1-8.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG
- Specificity
- HCMV gB
- Species Reactivity
- HCMV
- Clone
- h272.7
- Applications
- ELISA, WB, Neut
- Related Disease
- HCMV infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Cytomegalovirus gB; HCMV gB
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-443 | Recombinant Anti-HCMV GB Antibody | ELISA, WB, IP, FuncS | IgG |
| MHH-443 | Recombinant Human Anti-HCMV GB Antibody | FC, Neut, Funcs | IgG |
| FAMAB-0173CQ | Recombinant Mouse Anti-HCMV gB Antibody (7-17) | ELISA, Neut | Mouse IgG3, κ |
| HPAB-0639-CN | Recombinant Human Anti-HCMV gB Antibody (2F10) | ELISA, Neut | Human IgG |
| FAMAB-1777CQ | Human Anti-HCMV GB Recombinant Antibody (clone 5A6) | IP, Neut, FC, IF | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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