Mouse Anti-HDAC4 Recombinant Antibody (VS4-WK212)

Name: Mouse Anti-HDAC4 Recombinant Antibody (VS4-WK212)
SKU: VS4-WK212
Rating: 5 (1 reviews)

CAT#: VS4-WK212

This product is a mouse monoclonal antibody that recognizes HDAC4 and can be used in ELISA, WB, ICC.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line THP-1

Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm, nuclear speckles & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48723/1855_B7_15_cr5ad8881abe486_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_9_2_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48723/736_E10_1_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_B_9_5.jpg

Normal Tissue Figure 5 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_7_3.jpg

Normal Tissue Figure 6 Kidney

Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_9_5.jpg

Normal Tissue Figure 7 Testis

Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_6_6.jpg

Normal Tissue Figure 8 Skeletal muscle

Myocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_B_2_6.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71448/168654_A_9_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000068024-HDAC4

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Specifications

Immunogen
Recombinant fragment, corresponding to amino acids 16aa-225aa of human HDAC4 (N-terminus).

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG

Specificity
Human HDAC4

Species Reactivity
Human, Mouse, Rat

Applications
ELISA, WB, ICC

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, and 50% glycerol.

Preservative
0.2% sodium azide

Storage
Store at -20°C or -80°C. Avoid repeated freeze.

Shipping
Ice packs

Applications

Application Notes
Recommended dilution: WB:1:500-1:5000, ICC:1:50-1:200

Target

Alternative Names
Histone Deacetylase 4; EC 3.5.1.98; HD4; Brachydactyly-Mental Retardation Syndrome; Histone Deacetylase A; KIAA0288; HA6116;

Gene ID
9759

UniProt ID
P56524

Long Name
Histone Deacetylase 4

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Related Diseases

Alcoholism

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS4-WK212.pdf

MSDS

MSDS - VS4-WK212.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

See other products for "HDAC4"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOR-1576	Rabbit Anti-HDAC4 Recombinant Antibody (clone DS1576AB)	ICC, IF, WB	Rabbit IgG

CAT	Product Name	Application	Type
ZG-0415J	Mouse Anti-HDAC4 Recombinant Antibody (ZG-0415J)	WB, ELISA	Mouse IgG
ZG-0416J	Mouse Anti-HDAC4 Recombinant Antibody (ZG-0416J)	WB	Mouse IgG
ZG-0417J	Mouse Anti-HDAC4 Recombinant Antibody (ZG-0417J)	WB	Mouse IgG
VS3-QX506	Mouse Anti-HDAC4 Recombinant Antibody (clone 4A3-H7-C6)	WB, IP	Mouse IgG2a
VS3-QX507	Mouse Anti-HDAC4 Recombinant Antibody (clone 2E-6E)	WB, ICC, FC	Mouse IgG1

CAT	Product Name	Application	Type
VS-0724-YC1024	AbPlus™ Anti-HDAC4 Magnetic Beads (VS-0724-YC1024)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0325-XY1002	Anti-HDAC4 Immunohistochemistry Kit	IHC
VS-0525-XY3098	Anti-Human HDAC4 Immunohistochemistry Kit	IHC

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