Rabbit Anti-HIST1H3A Recombinant Antibody (VS13-YC517) (CAT#: VS13-YC517)
The Rabbit Anti-HIST1H3A Recombinant Antibody is specific for human, mouse and rat HIST1H3A. HIST1H3A belongs to the histone H3 family, encoding replication-dependent histones found in a gene cluster on chromosome 6 with palindromic termination elements instead of polyA tails. The anti-HIST1H3A antibody can be used for Western blot (1/20000), Immunocytochemistry (1/100), Immunofluorescence (1/100), Immunohistochemistry-paraffin (1/5000-1/10000), Immunoprecipitation (1-2 μg per 500 μL of cell lysate), Chromatin immunoprecipitation (0.5~5 μg per 25 μg of chromatin).
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_B_7_5.jpg
Figure 2 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_8_3.jpg
Figure 3 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_7_4.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_9_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000275714-HIST1H3A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000275714-HIST1H3A
Specifications
- Immunogen
- Recombinant human Histone H3, aa 85-136.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HIST1H3A
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, ChIP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/20000
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100
Immunohistochemistry-paraffin (IHC-P): 1/5000-1/10000
Immunoprecipitation (IP): 1-2 μg per 500 μL of cell lysate
Chromatin Immunoprecipitation (ChIP): 0.5~5 μg per 25 μg of chromatin
Target
- Alternative Names
- Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3/A; H3FA; HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J; Histone H3.1; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I;
- Gene ID
- 8350
- UniProt ID
- P68431
- Long Name
- Histone Cluster 1 H3 Family Member A
- Cellular Localization
- Nucleus; Chromosome
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1606 | Rabbit Anti-HIST1H3A Recombinant Antibody (clone DS1606AB) | ELISA | Rabbit IgG |
| MOR-4594 | Rabbit Anti-HIST1H3A Recombinant Antibody (clone TH107DS) | WB, IF, ICC, FC | Rabbit IgG |
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