Rabbit Anti-HIST1H3A Recombinant Antibody (VS13-YC517)
CAT#: VS13-YC517
The Rabbit Anti-HIST1H3A Recombinant Antibody is specific for human, mouse and rat HIST1H3A. HIST1H3A belongs to the histone H3 family, encoding replication-dependent histones found in a gene cluster on chromosome 6 with palindromic termination elements instead of polyA tails. The anti-HIST1H3A antibody can be used for Western blot (1/20000), Immunocytochemistry (1/100), Immunofluorescence (1/100), Immunohistochemistry-paraffin (1/5000-1/10000), Immunoprecipitation (1-2 μg per 500 μL of cell lysate), Chromatin immunoprecipitation (0.5~5 μg per 25 μg of chromatin).
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42570/103608_A_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000275714-HIST1H3A
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Specifications
- Immunogen
- Recombinant human Histone H3, aa 85-136.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HIST1H3A
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, ChIP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/20000
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100
Immunohistochemistry-paraffin (IHC-P): 1/5000-1/10000
Immunoprecipitation (IP): 1-2 μg per 500 μL of cell lysate
Chromatin Immunoprecipitation (ChIP): 0.5~5 μg per 25 μg of chromatin
Target
- Alternative Names
- Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3/A; H3FA; HIST1H3A HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J; Histone H3.1; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I;
- Gene ID
- 8350
- UniProt ID
- P68431
- Long Name
- Histone Cluster 1 H3 Family Member A
- Cellular Localization
- Nucleus; Chromosome
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HIST1H3A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1606 | Rabbit Anti-HIST1H3A Recombinant Antibody (clone DS1606AB) | ELISA | Rabbit IgG |
| MOR-4594 | Rabbit Anti-HIST1H3A Recombinant Antibody (clone TH107DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1815-CN | Rabbit Anti-HIST1H3A Polyclonal Antibody (MRO-1815-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2396-CN | Rabbit Anti-HIST1H3A Recombinant Antibody (clone JU43-26) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-2397-CN | Rabbit Anti-HIST1H3A Recombinant Antibody (clone CBACN-681) | WB, IF, IHC | Rabbit IgG |
| MRO-2404-CN | Rabbit Anti-HIST1H3A Recombinant Antibody (clone CBACN-687) | WB, IF, IHC | Rabbit IgG |
| MRO-2406-CN | Rabbit Anti-HIST1H3A Recombinant Antibody (clone CBACN-689) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC626 | AbPlus™ Anti-HIST1H3A Magnetic Beads (VS-0724-YC626) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1025 | Anti-HIST1H3A Immunohistochemistry Kit | IHC |
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