Rabbit Anti-HIST1H4A Recombinant Antibody (VS13-YC525)
CAT#: VS13-YC525
The Rabbit Anti-HIST1H4A Recombinant Antibody is specific for human, mouse and rat HIST1H4A. HIST1H4A, part of the histone H4 family, participates in chromatin structure, residing in a gene cluster on chromosome 6, characterized by intra-intronless transcripts lacking polyA tails. The anti-HIST1H4A antibody can be used for Western blot (1/500-1/5000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/200).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human liver
Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes and bile duct cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21887/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21887/48173_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21887/48173_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21887/48173_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21887/48173_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21887/48173_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21887/48173_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (MOLT-4, NB-4, REH)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000278637-HIST1H4A
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Specifications
- Immunogen
- Recombinant human Histone H4, aa 54-103.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HIST1H4A
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- Histone Cluster 1 H4 Family Member A; H4 Histone Family, Member A; Histone Cluster 1, H4a; Histone 1, H4a; H4FA; HIST1H4A HIST1H4B HIST1H4C HIST1H4D HIST1H4E HIST1H4F HIST1H4H HIST1H4I HIST1H4J HIST1H4K HIST1H4L HIST2H4A HIST2H4B HIST4H4; Histone H4; HIST2H4; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K;
- Gene ID
- 8359
- UniProt ID
- P62805
- Long Name
- Histone Cluster 1 H4 Family Member A
- Cellular Localization
- Nucleus; Chromosome
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HIST1H4A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3478z | Mouse Anti-HIST1H4A Recombinant Antibody (clone 40H9) | IHC, IP, WB | Mouse IgG1 |
| MRO-0750-CN | Rabbit Anti-HIST1H4A Recombinant Antibody (clone CBACN-276) | WB, IF, IHC | Rabbit IgG |
| MRO-2398-CN | Rabbit Anti-HIST1H4A Recombinant Antibody (clone JB21-44) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2399-CN | Rabbit Anti-HIST1H4A Recombinant Antibody (clone CBACN-682) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-2408-CN | Rabbit Anti-HIST1H4A Recombinant Antibody (clone CBACN-691) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1607 | Rabbit Anti-HIST1H4A Recombinant Antibody (clone DS1607AB) | WB, IP, IHC-P, IF, ChIP | Rabbit IgG |
| MOR-4597 | Rabbit Anti-HIST1H4A Recombinant Antibody (clone TH110DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-0047-FY | Rabbit Anti-HIST1H4A Recombinant Antibody (clone AFY0018) | IHC-P, IP, WB | Rabbit IgG |
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