Mouse Anti-Histone H1 Recombinant Antibody (clone 0725W19)

CAT#: VS-0725-FY175

The Mouse Anti-Histone H1 Recombinant Antibody (clone 0725W19) is a recombinant antibody developed against a recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. This antibody is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunofluorescence (IF), Immunoprecipitation (IP), and Western Blot (WB). Histone H1 plays a crucial role in compacting DNA into higher-order chromatin structures, influencing gene accessibility. This antibody offers insights into chromatin organization and its regulatory functions.

Specifications

  • Immunogen
  • A recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. Importantly, this region of the protein is not conserved in mammals, so the antibody is not expected to cross-react with human, mouse, or rat Histone H1.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Drosophila Histone H1
  • Species Reactivity
  • Drosophila
  • Clone
  • 0725W19
  • Applications
  • Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunofluorescence, Immunoprecipitation, Western Blot
  • Related Disease
  • Cancer, Developmental disorders, Neurodevelopmental disorders

Product Property

  • Purification
  • Protein A purified
  • Molecular Weight
  • The molecular weight of the antigen is 35 kDa.
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS, 30% glycerol
  • Preservative
  • 0.035% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 0.2-1 µg/mL

Target

  • Alternative Names
  • Histone H1
  • Long Name
  • Histone H1 (a linker histone)
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

Isotype Control

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