Mouse Anti-Histone H3 Recombinant Antibody (clone 0725W15)
CAT#: VS-0725-FY170
This recombinant antibody, clone 0725W15, targets the N-terminus of histone H3, using a peptide from this region as its immunogen. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). Histone H3 is a core component of the nucleosome, essential for chromatin structure and regulation of gene expression, DNA repair, replication, and chromosomal stability. This antibody serves as a valuable tool for investigating broad histone H3 functions.
Specifications
- Immunogen
- A peptide that includes the N-terminus of histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human Histone H3
- Species Reactivity
- Human
- Clone
- 0725W15
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Cancer, Chromosomal abnormalities, Developmental disorders, Neurodevelopmental disorders
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS pH 7.5, 30% glycerol, 0.3 M NaCl
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation-Sequencing: 4 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation: 5-10 µg per Chromatin Immunoprecipitation
Immunocytochemistry/Immunofluorescence: 1 µg/mL
Western Blot: 0.5-2 µg/mL
Target
- Alternative Names
- H3/A; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3
- Gene ID
- 8350
- UniProt ID
- P68431
- Long Name
- H3 clustered histone 1
- Sequence Similarities
- Belongs to the histone H3 family.
- Cellular Localization
- Chromosome, Nucleosome core, Nucleus
- Post Translation Modifications
- Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.
- Protein Refseq
- NP_003520.1; NP_003521.2; NP_003522.1
- Function
- Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0725-FY170. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Histone H3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-089CT | Human Anti-Histone H3 Recombinant Antibody (TAB-089CT) | ELISA, WB | Humanized IgG |
| TAB-089CT-S(P) | Human Anti-Histone H3 Recombinant Antibody; scFv Fragment (TAB-089CT-S(P)) | ELISA, WB | Humanized scFv |
| TAB-089CT-F(E) | Human Anti-Histone H3 Recombinant Antibody; Fab Fragment (TAB-089CT-F(E)) | ELISA, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4522 | Rabbit Anti-Histone H3 Recombinant Antibody (clone TH30DS) | WB, IF, ICC, FC, ChIP | Rabbit IgG |
| MOR-4591 | Rabbit Anti-Histone H3 Recombinant Antibody (clone TH104DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-4592 | Rabbit Anti-Histone H3 Recombinant Antibody (clone TH105DS) | WB, IF, ICC, ChIP, ELISA | Rabbit IgG |
| MOR-4647 | Rabbit Anti-Histone H3 Recombinant Antibody (clone TH160DS) | WB, IF, ICC, FC, ChIP | Rabbit IgG |
| MOR-4754 | Rabbit Anti-Histone H3 Recombinant Antibody (clone TH268DS) | WB, FC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0205YJ | Rabbit Anti-Histone H3 Polyclonal Antibody | WB, IHC, IF, ICC, ELISA | Rabbit IgG |
| MRO-0228YJ | Mouse Anti-Histone H3 Recombinant Antibody (clone CBS173) | WB, IF, ICC, IP, ELISA | Mouse IgG |
| FAMAB-1364CQ | Mouse Anti-Histone H3 Recombinant Antibody (clone 9E4) | IB, ELISA | Mouse IgG1 |
| VS1-YJ90 | Mouse Anti-Histone H3 Recombinant Antibody (clone 1B1-B2) | IB, IP | Mouse IgG3, κ |
| VS4-WK217 | Mouse Anti-Histone H3 Recombinant Antibody (VS4-WK217) | ELISA, WB | Mouse IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, WB, FC, IHC, IP
-2.png)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
