Mouse Anti-Histone H3 Recombinant Antibody (clone 1G1)
CAT#: VS7-0425-WR10
This product is a recombinant mouse IgG antibody that binds human Histone H3. 1G1 can be used for WB, IHC-P, IF, ICC, and IP assays. It can also cross reacts with mouse, rat, and yeast.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42570/103608_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000275714-H3C1
❮
❯
Specifications
- Immunogen
- Recombinant human H31 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human H31
- Species Reactivity
- Human, Mouse, Rat, Yeast
- Clone
- 1G1
- Molecule Class
- Structure Molecule
- Applications
- WB, IHC-P, IF, ICC, IP
- Conjugate
- Unconjugated
- Related Disease
- Cancer; Developmental disorders
Product Property
- Clonality
- Monoclonal
- Purification
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunofluorescence: 1:100-500
Immunohistochemistry: 1:50-300
Immunoprecipitation: 1:200
Western Blot: 1:2000-5000
Target
- Alternative Names
- H3.1; H3/d; H3C1; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD; H3C10; H3C11; H3C12; HIST1H3E
- Long Name
- Histone H3.1
- Cellular Localization
- Chromosome; Nucleosome core; Nucleus
- Post Translation Modifications
- Acetylation; ADP-ribosylation; Citrullination; Hydroxylation; Lipoprotein; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_066298
- Function
- Primate-specific variant histone H3, which constitutes a core component of nucleosomes (PubMed:20819935, PubMed:27016736).; Histone H3.Y-containing nucleosomes accumulate around transcription start sites and have flexible DNA ends, suggesting that they form relaxed chromatin that allows transcription factor access (PubMed:27016736).; Histone H1 binds less efficiently to histone H3.Y-containing nucleosomes (PubMed:27016736).; Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (Probable)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "Clone 1G1"
- CAT
- Product Name
See other products for "H3C1"
Select a product category from the dropdown menu below to view related products.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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