Mouse Anti-Histone H3 Recombinant Antibody (clone 4A1) (CAT#: FAMAB-1367CQ)

This product is a recombinant mouse antibody (clone 4A1) that recognizes Histone H3. 4A1 was purified by affinity chromatography. It can be used in IB, ELISA.


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IB and ELISA

Figure 1 Immunoreactivity of IgE mAbs to histone H3.

Figure 1 Immunoreactivity of IgE mAbs to histone H3.

(a) Bovine total histones (5 ug) and histone H3 (0.75 ug) were separated on an SDS-PAGE under reducing condition followed by immunoblotting using IgE mAbs and an isotype control. (b) Immunoreactivity of IgE mAbs (1 ug/mL) to bovine histone H3 was also measured by ELISA. Anti-DNP specific IgE mAb, 2-9(4), was used as an isotype control. mAb, monoclonal antibody.

Matsubara, T., Aoki, N., Hino, S., Okajima, T., Nadano, D., & Matsuda, T. (2009). Serum and monoclonal immunoglobulin E antibodies from NC/Nga mice with severe atopic‐like dermatitis recognize an auto‐antigen, histone H3. Clinical & Experimental Allergy, 39(4), 579-590.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Mouse histone H3, and cross-reacts with bovine H3.
  • Species Reactivity
  • Bovine, Mouse
  • Clone
  • 4A1
  • Applications
  • IB, ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • HIST3H3; H3t; H3.4; H3/g; H3FT

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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