Mouse Anti-Histone H3K27me1 Recombinant Antibody (clone 0725W3)
CAT#: VS-0725-FY156
This recombinant antibody, clone 0725W3, targets monomethyl Lys27 of human Histone H3, developed using a peptide containing this specific modification. It is validated for various applications, including Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). Histone H3, a core component of the nucleosome, is crucial for chromatin structure. This antibody provides a valuable tool for researchers investigating the role of histone methylation in gene regulation and chromatin dynamics.
Specifications
- Immunogen
- A peptide containing monomethyl Lys27 from human Histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human Histone H3K27me1
- Species Reactivity
- Human
- Clone
- 0725W3
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Cleavage Under Targets & Tagmentation, Dot Blot, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Cancer
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS pH 7.5, 30% glycerol, 0.3 M NaCl
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 2-5 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 2-5 µg each
Western Blot: 0.5-2 µg/mL
Cleavage Under Targets & Tagmentation: 1-2 µg per 50 µL reaction
Target
- Alternative Names
- Histone H3K27me1
- Long Name
- Histone H3 Lysine 27 monomethylation
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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