Mouse Anti-Histone H3K27me2 Recombinant Antibody (clone 0725W6)
CAT#: VS-0725-FY159
The Mouse Anti-Histone H3K27me2 Recombinant Antibody (clone 0725W6) is a recombinant product developed to target dimethyl Lysine 27 of human histone H3, utilizing a synthetic peptide with this specific modification. It is validated for use in Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), and Western Blot (WB). Histone H3 forms the core of the nucleosome, the basic subunit of chromatin, making this antibody invaluable for exploring the roles of histone modifications in chromatin organization and gene expression.
Specifications
- Immunogen
- A synthetic peptide containing dimethyl Lysine 27 of human histone H3.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human Histone H3K27me2
- Species Reactivity
- Human
- Clone
- 0725W6
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Cleavage Under Targets & Tagmentation, Western Blot
- Related Disease
- Glioma (specifically H3K27M mutant glioma)
Product Property
- Molecular Weight
- The molecular weight of the antigen is 17 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS pH 7.5, 30% glycerol, 0.3 M NaCl
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 2 µg per Chromatin Immunoprecipitation
Western Blot: 0.5-2 µg/mL
Cleavage Under Targets & Tagmentation: 1-2 µg per 50 µL reaction
Target
- Alternative Names
- Histone H3K27me2
- Long Name
- Histone H3 Lysine 27 dimethylation
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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