Human Anti-HIV-1 Env Recombinant Antibody (clone PGDM1401) (CAT#: MRO-2782CQ)

Recombinant human antibody to Env. PGDM1401 had exceptionally broad (65% against a 77-virus panel) and potent (median IC50, 0.005 µg/ml) cross-clade neutralization coverage. PGDM1401 can bind monomeric gp120 and is autoreactive in HEp2 assays.


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Neu

Figure 1 Despite differences in neutralization, somatic variants bind to the same region on Env.

Figure 1 Despite differences in neutralization, somatic variants bind to the same region on Env.

The somatic variants PGDM1400–1407 all bind to the BG505 SOSIP.664-AviB construct by ELISA.

Sok, D., van Gils, M. J., Pauthner, M., Julien, J. P., Saye-Francisco, K. L., Hsueh, J., ... & Hua, Y. (2014). Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex. Proceedings of the National Academy of Sciences, 111(49), 17624-17629.

FC

Figure 2 Somatic variants bind to cell-surface BG505 Env despite not neutralizing the BG505 pseudovirus.

Figure 2 Somatic variants bind to cell-surface BG505 Env despite not neutralizing the BG505 pseudovirus.

Somatic variants were tested for binding to BG505 Env expressed on the surface of 293T cells. Antibody PGT151, which binds specifically to cleaved Env, and antibody b6, which binds to uncleaved Env, were included for comparison.

SPR

Figure 3 Binding of mAbs to BG505 SOSIP.664-aviB by Octet.

Figure 3 Binding of mAbs to BG505 SOSIP.664-aviB by Octet.

Antibodies PGT145, PGDM1400, PGDM1401, PGDM1403, PGDM1405, and PGDM1407 were analyzed for binding to BG505 SOSIP.664-AviB by Octet.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Recognizes HIV-1 Env
  • Species Reactivity
  • HIV-1
  • Clone
  • PGDM1401
  • Applications
  • Neut, ELISA, FC, SPR

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The HIV-1 Env antibody has been reported in applications of Neutralization, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Surface Plasmon Resonance.

Target

  • Alternative Names
  • ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Recombinant Antibody

Single-domain Antibody

CAT Product Name Application Type
NABG-057 Recombinant Anti-HIV-1 env VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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