Recombinant human antibody to Env. PGDM1401 had exceptionally broad (65% against a 77-virus panel) and potent (median IC50, 0.005 µg/ml) cross-clade neutralization coverage. PGDM1401 can bind monomeric gp120 and is autoreactive in HEp2 assays.
Figure 1 Despite differences in neutralization, somatic variants bind to the same region on Env.
The somatic variants PGDM1400–1407 all bind to the BG505 SOSIP.664-AviB construct by ELISA.
Sok, D., van Gils, M. J., Pauthner, M., Julien, J. P., Saye-Francisco, K. L., Hsueh, J., ... & Hua, Y. (2014). Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex. Proceedings of the National Academy of Sciences, 111(49), 17624-17629.
Figure 2 Somatic variants bind to cell-surface BG505 Env despite not neutralizing the BG505 pseudovirus.
Somatic variants were tested for binding to BG505 Env expressed on the surface of 293T cells. Antibody PGT151, which binds specifically to cleaved Env, and antibody b6, which binds to uncleaved Env, were included for comparison.
Figure 3 Binding of mAbs to BG505 SOSIP.664-aviB by Octet.
Antibodies PGT145, PGDM1400, PGDM1401, PGDM1403, PGDM1405, and PGDM1407 were analyzed for binding to BG505 SOSIP.664-AviB by Octet.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-474 | Recombinant Anti-HIV-1 Env Antibody | ELISA, WB, IF, FuncS | IgG |
MHH-474 | Recombinant Human Anti-HIV-1 Env Antibody | WB, FuncS | IgG |
PABL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124) | WB, Neut, FuncS | Human IgG |
PABL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22) | WB, ELISA, Neut, FuncS | Human IgG |
PABL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L) | WB, ELISA, IF, FuncS | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124); Fab Fragment | WB, Neut, FuncS | Human Fab |
PFBL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22); Fab Fragment | WB, ELISA, Neut, FuncS | Human Fab |
PFBL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
PFBL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11); Fab Fragment | WB, ELISA, FuncS | Human Fab |
PFBL-145 | Human Anti-HIV-1 Env Recombinant Antibody; Fab Fragment (PFBL-145) | ELISA, Neut, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-2802CQ-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone F285); scFv Fragment | ELISA | Human scFv |
MRO-2803CQ-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone VRC01b); scFv Fragment | ELISA, Neut | Human scFv |
PSBC-420 | Human Anti-HIV-1 Env Recombinant Antibody (clone CAP228-3D); scFv Fragment | Neut | Human scFv |
PABJ-0039-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone 1-55); scFv Fragment | ELISA, Neut | Human scFv |
PABJ-0053-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone 1-18); scFv Fragment | ELISA, Neut | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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