Human Anti-HIV-1 Env Recombinant Antibody (clone N3C5) (CAT#: MRO-2810CQ)
Recombinant Humanized Antibody to Env. N3C5 was shown to bind to both homologous gp140 and heterologous gp140 from clades C and A with high affinity, but not to clade B. It was shown to bind conformational epitope within the immunodominant region of gp41
Specific Inquiry
ELISA
Figure 1 Binding of monomeric and pentameric forms of N3C5 and N03B11 to gp14097CN54.
Pooled fractions from the 900- and 180-kDa fractions after liquid chromatography were tested by ELISA at 0.05 μg/ ml (N3C5) and 1 μg/ml (N03B11).
Sheppard, N. C., Davies, S. L., Jeffs, S. A., Vieira, S. M., & Sattentau, Q. J. (2007). Production and characterization of high-affinity human monoclonal antibodies to human immunodeficiency virus type 1 envelope glycoproteins in a mouse model expressing human immunoglobulins. Clin. Vaccine Immunol., 14(2), 157-167.
ELISA
Figure 2 Defining the epitopes of MAbs N3C5 and N03B11.
Binding of MAbs N3C5 and N03B11 to native and denatured gp14097CN54. Squares represent N3C5 and triangles represent N03B11.
Sheppard, N. C., Davies, S. L., Jeffs, S. A., Vieira, S. M., & Sattentau, Q. J. (2007). Production and characterization of high-affinity human monoclonal antibodies to human immunodeficiency virus type 1 envelope glycoproteins in a mouse model expressing human immunoglobulins. Clin. Vaccine Immunol., 14(2), 157-167.
Inhib
Figure 3 Defining the epitopes of MAbs N3C5 and N03B11.
Reciprocal MAb competition studies by SPR and ELISA.
Sheppard, N. C., Davies, S. L., Jeffs, S. A., Vieira, S. M., & Sattentau, Q. J. (2007). Production and characterization of high-affinity human monoclonal antibodies to human immunodeficiency virus type 1 envelope glycoproteins in a mouse model expressing human immunoglobulins. Clin. Vaccine Immunol., 14(2), 157-167.
Neu
Figure 4 The neutralization of HIV-197CN54 by IgM MAbs N3C5 and N03B11.
Bars represent percent neutralization compared to an equal concentration of an irrelevant Ab (D7324). The MAbs were also combined with heat-inactivated (HI) or fresh (C) serum (NHS) to assess CMI. N3C5 and N03B11 were used at 100 μg/ml while the positive control, MAb 447-52D, was used at 10 μg/ml 1.
Sheppard, N. C., Davies, S. L., Jeffs, S. A., Vieira, S. M., & Sattentau, Q. J. (2007). Production and characterization of high-affinity human monoclonal antibodies to human immunodeficiency virus type 1 envelope glycoproteins in a mouse model expressingontrol, MAb 447-52D, was used at 10 μg/ml 1.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgM
- Specificity
- Recognizes HIV-1 Env
- Species Reactivity
- HIV-1
- Clone
- N3C5
- Applications
- ELISA, Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Env antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Inhibition.
ELISA: 0.05 μg/ml.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124); Fab Fragment | WB, Neut, FuncS | Human Fab |
| PFBL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22); Fab Fragment | WB, ELISA, Neut, FuncS | Human Fab |
| PFBL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
| PFBL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| PFBL-145 | Human Anti-HIV-1 Env Recombinant Antibody; Fab Fragment (PFBL-145) | ELISA, Neut, FuncS | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-514 | Human Anti-HIV-1 Env Recombinant Antibody (clone 17B) | FC, Neut | Human IgG1 |
| PABL-516 | Monkey Anti-HIV-1 Env Recombinant Antibody (clone GE356) | WB, ELISA, FuncS | Monkey IgG |
| PABL-519 | Human Anti-HIV-1 Env Recombinant Antibody (PABL-519) | Neut | Human IgG |
| MRO-781CQ | Mouse Anti-HIV-1 Env Recombinant Antibody (clone 3H9/D6) | ELISA, WB | Mouse IgG |
| MRO-2742CQ | Human Anti-HIV-1 Env Recombinant Antibody (clone 10.9D) | ELISA | Human IgG |
MHC Tetramer for Virology
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2324LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2324LY-S(P)) | Neut, ELISA | Mouse scFv |
| HPAB-2325LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2325LY-S(P)) | Neut, ELISA | Mouse scFv |
| HPAB-2326LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2326LY-S(P)) | Neut, ELISA | Mouse scFv |
| HPAB-2327LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2327LY-S(P)) | Neut, ELISA | Mouse scFv |
| HPAB-0122-WJ-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone B358.II); scFv Fragment | ELISA, WB | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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