Mouse Anti-HIV-1 Env Recombinant Antibody (clone 15E9) (CAT#: MRO-2814CQ)
Recombinant mouse antibody to Env. 15E9 weakly cross-reacted with all of the tested HIV-1 subtypes and with SIVcpz in Enzyme-linked Immunosorbent Assay but only reacted with one subtype A in Western blot.
Specific Inquiry
WB
Figure 1 Cross clade reactivity of mAbs to a panel of divers HIV-1 envelope proteins.
HIV-1 Env proteins (100 ng/each) from five subtypes (A, B, C, D and F), a circulating recombinant (CRF01_AE), a group M consensus (CON), and an SIVcpzUS1 (SIV) was tested with newly characterized mAbs.
Gao, F., Scearce, R. M., Alam, S. M., Hora, B., Xia, S., Hohm, J. E., ... & Lomas, W. E. (2009). Cross-reactive monoclonal antibodies to multiple HIV-1 subtype and SIVcpz envelope glycoproteins. Virology, 394(1), 91-98.
SPR
Figure 2 Binding kinetics analysis of mAbs to HIV-1 Env proteins by Surface Plasmon Resonance.
HIV-1 Env gp140 proteins were immobilized to about 1000 RU using amine coupling chemistry. Positive control V3 mAb F39F was used to assess activity of gp140 proteins following immobilization. Monoclonal Abs were injected at 50 μg/mL for 2 min at 30 ul/min. For binding kinetics analyses, mAbs were injected at varying concentrations ranging from 1.2 mg/mL to 1.5 μg/ml.
Gao, F., Scearce, R. M., Alam, S. M., Hora, B., Xia, S., Hohm, J. E., ... & Lomas, W. E. (2009). Cross-reactive monoclonal antibodies to multiple HIV-1 subtype and SIVcpz envelope glycoproteins. Virology, 394(1), 91-98.
Specifications
- Immunogen
- Subtype C Env protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Recognizes HIV-1 Env
- Species Reactivity
- HIV-1
- Clone
- 15E9
- Applications
- WB, SPR
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Env antibody has been reported in applications of Western Blot, Surface Plasmon Resonance.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124) | WB, Neut, FuncS | Human IgG |
| PABL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L) | WB, ELISA, IF, FuncS | Human IgG |
| PABL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11) | WB, ELISA, FuncS | Human IgG |
| PABL-145 | Human Anti-HIV-1 Env Recombinant Antibody (PABL-145) | ELISA, Neut, FuncS | Human IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-511 | Human Anti-HIV-1 Env Recombinant Antibody (clone 17B); Fab Fragment | Neut | Human Fab |
| PFBL-512 | Recombinant Human Anti-HIV-1 Env Antibody Fab Fragment (A32) | WB, FuncS | Fab |
| PFBL-513 | Monkey Anti-HIV-1 Env Recombinant Antibody (clone GE356); Fab Fragment | WB, FuncS | Monkey Fab |
| PFBL-516 | Human Anti-HIV-1 Env Recombinant Antibody; Fab Fragment (PFBL-516) | Neut | Human Fab |
| MRO-2742CQ-F(E) | Human Anti-HIV-1 Env Recombinant Antibody (clone 10.9D); Fab Fragment | ELISA | Human Fab |
MHC Tetramer for Virology
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-321 | Human Anti-HIV-1 Env Recombinant Antibody (clone PCT64-13C); scFv Fragment | Neut | Human scFv |
| PABJ-0103-S(P) | Rabbit Anti-HIV-1 Env Recombinant Antibody (clone E70); scFv Fragment | ELISA, Neut | Rabbit scFv |
| PABJ-0140-S(P) | Human Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (PABJ-0140-S(P)) | ELISA, Neut | Human scFv |
| PABJ-0141-S(P) | Human Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (PABJ-0141-S(P)) | ELISA, Neut | Human scFv |
| PABJ-0147-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone ACS202); scFv Fragment | ELISA, Neut | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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