Human Anti-HIV-1 gp120 Recombinant Antibody (clone 2C6) (CAT#: MRO-3022CQ)

Figure 1 MAb 2C6 binding recapitulates B cell clonal supernatant screening ELISA results.

Binding specificity of mAb 2C6 and control mAb RV6-26 expressed as Fabs against the following HIV antigens were assayed by ELISA: BaL gp120 monomer, Gag VLPs, and Gag-Env-VLPs (parental strain of Env was BaL).

Hicar, M. D., Chen, X., Briney, B., Hammonds, J., Wang, J. J., Kalams, S., ... & Crowe Jr, J. E. (2010). Pseudovirion particles bearing native HIV envelope trimers facilitate a novel method for generating human neutralizing monoclonal antibodies against HIV. Journal of acquired immune deficiency syndromes (1999), 54(3), 223.

Figure 2 Neutralization of Fab 2C6 compared to known CD4i mAb 17b.

HIV strain BaL was incubated for 90 min at room temperature with Abs (12.5 µg/ml) in theabsence or presence of 0.075 µg/ml of soluble CD4. Neutralization was detected 48 hours later. HIV immune patient serum was used as a positive control.

Hicar, M. D., Chen, X., Briney, B., Hammonds, J., Wang, J. J., Kalams, S., ... & Crowe Jr, J. E. (2010). Pseudovirion particles bearing native HIV envelope trimers facilitate a novel method for generating human neutralizing monoclonal antibodies against HIV. Journal of acquired immune deficiency syndromes (1999), 54(3), 223.