Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH386) (CAT#: MRO-3087CQ)

Figure 1 Inhibition of HIV-1 epithelial cell binding and dendritic cell (DC)-mediated trans-infection by envelope (Env)-specific colostrum monoclonal antibodies (mAbs).

Of the 15 colostrum gp120-specific antibodies, 6 inhibit DC transfer of clade C HIV-1 variant MW965 from mature DCs to CD4 þ / CCR5 þ target cells (TZM-bl). The cutoff for significant response in both assays was determined by the mean response of the negative control (anti-influenza mAb CH65) plus 2 s.d. (dotted line).

Jeffries, T. L., Sacha, C. R., Pollara, J., Himes, J., Jaeger, F. H., Dennison, S. M., ... & LaBranche, C. (2016). The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells. Mucosal immunology, 9(2), 414-427.

Figure 2 HIV-1 gp120-specific colostrum monoclonal antibodies (mAbs) are crossreactive with commensal bacteria whole-cell lysate (WCL) by western blot, including specific reactivity against Escherichia coli Chaperonin 60.

The gp120-specific mAbs were tested for reactivity to bacterial WCL by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) reduced and nonreduced western blot. The mAb DH386 was nonreactive (-) to gut microbiota WCL.

Jeffries, T. L., Sacha, C. R., Pollara, J., Himes, J., Jaeger, F. H., Dennison, S. M., ... & LaBranche, C. (2016). The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells. Mucosal immunology, 9(2), 414-427.