Mouse Anti-HIV-1 Nef Recombinant Antibody (clone A7) (CAT#: MRO-4213CQ)
Recombinant mouse antibody to Nef(28-45). A7 can bind to the peptide spanning amino acids 28-45.
Specific Inquiry
ELISA
Figure 1 Recognition of Env proteins from different constructs by the selected scFv.
Binding of the two antibody classes scFv A7 and
A2 to gp140 and gp120 Env proteins determined by ELISA.
Trott, M., Weiß, S., Antoni, S., Koch, J., Von Briesen, H., Hust, M., & Dietrich, U. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3-and a trimer-specific gp41 antibody. PLoS One, 9(5).
ELISA
Figure 2 Analysis scFv-Fc A2 and A7 for autoreactivity against cardiolipin and phosphatidylserine.
Autoreactivity of purified scFvFc A2 and A7 was analyzed by commercial ELISA using the internal positive and negative control sera.
Trott, M., Weiß, S., Antoni, S., Koch, J., Von Briesen, H., Hust, M., & Dietrich, U. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3-and a trimer-specific gp41 antibody. PLoS One, 9(5).
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Recognizes HIV-1 Nef(28-45)
- Species Reactivity
- HIV-1
- Clone
- A7
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Nef antibody has been reported in applications of Enzyme-linked Immunosorbent Assay. ELISA: Detection was with anti c-myc antibody (300 ng/well) and HRP conjugated anti-mouse
antibody (1:1,000).
Target
- Alternative Names
- HIV Nef; Human Immunodeficiency Viruse Nef; Human Immunodeficiency Viruse; HIV Nef protein; HIV Nef-protein; p27; HIV1gp9; F-protein; Negative factor
- Gene ID
- 156110
- UniProt ID
- P04601
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone A7"
See other products for "HIV-1 Nef"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-013 | Recombinant Anti-HIV nef VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-562 | Human Anti-HIV-1 Nef Recombinant Antibody (clone C1-28); scFv Fragment | FC | Human scFv |
| PSBL-563 | Human Anti-HIV-1 Nef Recombinant Antibody (clone H27-14); scFv Fragment | FC | Human scFv |
| MRO-4225CQ-S(P) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone NF2B2); scFv Fragment | ELISA | Mouse scFv |
| MRO-4226CQ-S(P) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone NF3A3); scFv Fragment | ELISA | Mouse scFv |
| MRO-4227CQ-S(P) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone NF8B4); scFv Fragment | ELISA | Mouse scFv |
MHC Tetramer for Virology
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-4205CQ | Human Anti-HIV-1 Nef Recombinant Antibody (clone 3B4B) | ELISA | Human IgM |
| MRO-4206CQ | Human Anti-HIV-1 Nef Recombinant Antibody (clone 4H4) | ELISA, IF, FC | Human IgG1 |
| MRO-4207CQ | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2A3) | ELISA, WB, IHC | Mouse IgG1 |
| MRO-4208CQ | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2EA) | ELISA, WB, IHC | Mouse IgG1 |
| MRO-4211CQ | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 3G12) | ELISA, WB, IHC | Mouse IgG2a |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-4205CQ-F(E) | Human Anti-HIV-1 Nef Recombinant Antibody (clone 3B4B); Fab Fragment | ELISA | Human Fab |
| MRO-4206CQ-F(E) | Human Anti-HIV-1 Nef Recombinant Antibody (clone 4H4); Fab Fragment | ELISA, IF, FC | Human Fab |
| MRO-4207CQ-F(E) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2A3); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| MRO-4208CQ-F(E) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2EA); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| MRO-4209CQ-F(E) | Mouse Anti-HIV-1 Nef Recombinant Antibody (clone 2F2); Fab Fragment | ELISA, WB | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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