Mouse Anti-HIV-1 p24 Recombinant Antibody (clone BI1279) (CAT#: FAMAB-0183CQ)

This product is a recombinant mouse antibody clone BI1279, which specifically binds to amino-terminal residues.


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WB

Figure 1 Western blot analysis using mAbs to probe HIV-l lysate strips.

Figure 1 Western blot analysis using mAbs to probe HIV-l lysate strips.

HIV-I viral lysate proteins were electrophoresed on a 12% SD-polyacryiamide gel and transferred to a membrane from which identical strips were prepared. Each strip was probed with 1 μg/ml of the mAb indicated, then incubated with alkaline phosphatase-conjugated goat anti-mouse IgG, and developed. Molecular weights (in kilodaltons) of protein markers run on the same gel are indicated with arrows.

Stevens, P. W., Hansen, C., & Jolley, M. E. (1993). Monoclonal antibodies to HIV-1 p24 core protein include pairs which exhibit synergistic binding. Molecular immunology, 30(3), 243-254.

Figure 2 Simultaneous competition assays using PCFIA.

Figure 2 Simultaneous competition assays using PCFIA.

A constant amount of fluorescein-labeled mAb was bound to excess antigen (HIV-I lysate) in the presence of serial 2-fold dilutions of unlabeled competing mAb. The fluorescein-labeled mAb is used at a concn corresponding to approximately half its saturation value in a PCFIA direct binding assay, while the highest concn of unlabeled mAb corresponds to its near-saturation value in a PCFIA direct binding assay. Values plotted are means of values from triplicate assays. Symbols for the unlabeled mAbs in all graphs are: ○ BI1279, ● BB128, + BB328, × BB223, □ BB344, ■ B11710, and ∆ BI1777. Fluorescein-labeled mAbs are (A) BI1279; (B) BB128; (C) BB223; (D) BB344; (E) BI1710; and (F) BI1777.

Stevens, P. W., Hansen, C., & Jolley, M. E. (1993). Monoclonal antibodies to HIV-1 p24 core protein include pairs which exhibit synergistic binding. Molecular immunology, 30(3), 243-254.


Specifications

  • Immunogen
  • Purified HIV-1 viral lysate
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • HIV-1 p24
  • Species Reactivity
  • HIV-1
  • Clone
  • BI1279
  • Applications
  • ELISA, WB
  • Related Disease
  • HIV-1 infection

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot.

Target

  • Alternative Names
  • Human immunodeficiency virus type 1; HIV-1; Capsid protein p24; p24

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone BI1279"

See other products for "HIV-1 p24"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1613 Rabbit Anti-HIV-1 p24 Recombinant Antibody (clone DS1613AB) WB Rabbit IgG

Single-domain Antibody

CAT Product Name Application Type
PABC-580 Recombinant Llama Anti-HIV-1 p24 Single Domain Antibody (VHH 59H10) ELISA Llama VHH

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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