Mouse Anti-HIV-1 pol Recombinant Antibody (clone a4 (Maba4)) (CAT#: MRO-4263CQ)
Recombinant mouse antibody to Pol(862-881). a4 recognized the N-terminal portion SQGVVESMNKELKKIIGQVR of K159 (pep-a4).
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ELISA
Figure 1 Identification of the epitope and residues that bind MAba4.
The histogram represents the ELISA results provided by the binding of
the MAba4 to the immunizing peptide K159, shorter and longer peptides, analogues and IN.
Azzi, S., Parissi, V., Maroun, R. G., Eid, P., Mauffret, O., & Fermandjian, S. (2010). The HIV-1 Integrase a4-Helix Involved in LTR-DNA Recognition Is also a Highly Antigenic Peptide Element.
WB
Figure 2 Standard concerted integration assays were performed with 1 pmole of IN in presence of increasing amounts of MAba4.
The final NaCl concentration was adjusted to 30 mM.
Azzi, S., Parissi, V., Maroun, R. G., Eid, P., Mauffret, O., & Fermandjian, S. (2010). The HIV-1 Integrase a4-Helix Involved in LTR-DNA Recognition Is also a Highly Antigenic Peptide Element.
Inhib
Figure 3 Inhibition assays were performed under different preincubation conditions.
MAba4 was either added simultaneously to
IN and DNA ([IN+DNA+MAba4]), either after preincubation between IN and DNA ([IN+DNA]+MAba4) or it was preincubated with IN before adding the
DNA substrates ([IN+MAba4]+DNA).
Azzi, S., Parissi, V., Maroun, R. G., Eid, P., Mauffret, O., & Fermandjian, S. (2010). The HIV-1 Integrase a4-Helix Involved in LTR-DNA Recognition Is also a Highly Antigenic Peptide Element.
Specifications
- Immunogen
- IN peptide K159
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Recognizes HIV-1 Pol(862-881)
- Species Reactivity
- HIV-1
- Clone
- a4 (Maba4)
- Applications
- ELISA, WB, Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 pol antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western blot, Inhibition. WB: MAba4 was added to the mixture at different concentrations and
the reaction products were loaded on a 1% agarose gel: 0 (lane 1), 10 (lane 2), 20 (lane 3), 30 (lane 4), 40 (lane 5), 50 (lane 6), 100 (lane 7), 200 lane (8),
600 (lane 9) or 800 ng (lane 10).
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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