Mouse Anti-HIV-1 pol Recombinant Antibody (clone a4 (Maba4)) (CAT#: MRO-4263CQ)

Figure 1 Identification of the epitope and residues that bind MAba4.

The histogram represents the ELISA results provided by the binding of
the MAba4 to the immunizing peptide K159, shorter and longer peptides, analogues and IN.

Azzi, S., Parissi, V., Maroun, R. G., Eid, P., Mauffret, O., & Fermandjian, S. (2010). The HIV-1 Integrase a4-Helix Involved in LTR-DNA Recognition Is also a Highly Antigenic Peptide Element.

Figure 2 Standard concerted integration assays were performed with 1 pmole of IN in presence of increasing amounts of MAba4.

The final NaCl concentration was adjusted to 30 mM.

Azzi, S., Parissi, V., Maroun, R. G., Eid, P., Mauffret, O., & Fermandjian, S. (2010). The HIV-1 Integrase a4-Helix Involved in LTR-DNA Recognition Is also a Highly Antigenic Peptide Element.

Figure 3 Inhibition assays were performed under different preincubation conditions.

MAba4 was either added simultaneously to
IN and DNA ([IN+DNA+MAba4]), either after preincubation between IN and DNA ([IN+DNA]+MAba4) or it was preincubated with IN before adding the
DNA substrates ([IN+MAba4]+DNA).

Azzi, S., Parissi, V., Maroun, R. G., Eid, P., Mauffret, O., & Fermandjian, S. (2010). The HIV-1 Integrase a4-Helix Involved in LTR-DNA Recognition Is also a Highly Antigenic Peptide Element.