Mouse Anti-HIV-1 pol Recombinant Antibody (clone MAb 35) (CAT#: MRO-4266CQ)

Figure 1 Neutralization of MuLV by SU-specific MAbs.

Neutralization assays for the indicated MAbs were performed with MuLV-luciferase-encoding virions.

Burkhart, M. D., Kayman, S. C., He, Y., & Pinter, A. (2003). Distinct mechanisms of neutralization by monoclonal antibodies specific for sites in the N-terminal or C-terminal domain of murine leukemia virus SU. Journal of virology, 77(7), 3993-4003.

Figure 2 Inhibition of MuLV SU binding to mCAT-1 by MAbs.

MuLV pseudotyped with the Friend/AKR 393/426 chimeric Env was incubated with 293 or 293.mCAT cells for 1 h at 37°C, and
virion binding was detected by FACS.

Burkhart, M. D., Kayman, S. C., He, Y., & Pinter, A. (2003). Distinct mechanisms of neutralization by monoclonal antibodies specific for sites in the N-terminal or C-terminal domain of murine leukemia virus SU. Journal of virology, 77(7), 3993-4003.

Figure 3 Viral supernatant from NIH 3T3 cells infected with MuLV bearing the Friend/ AKR 393/426 Env was incubated with or without MAbs for 1 h at 37°C.

After fixation and staining of syncytia, fused nuclei were counted in four
equal areas for each data point, and this number was used to calculate
a percent inhibition relative to no antibody treatment.

Burkhart, M. D., Kayman, S. C., He, Y., & Pinter, A. (2003). Distinct mechanisms of neutralization by monoclonal antibodies specific for sites in the N-terminal or C-terminal domain of murine leukemia virus SU. Journal of virology, 77(7), 3993-4003.

Figure 4 Precipitation of intact virions with Env-specific MAbs.

Culture supernatants from NIH 3T3 cells infected with MuLV bearing the
chimeric Friend/AKR 393/426 Env .

Burkhart, M. D., Kayman, S. C., He, Y., & Pinter, A. (2003). Distinct mechanisms of neutralization by monoclonal antibodies specific for sites in the N-terminal or C-terminal domain of murine leukemia virus SU. Journal of virology, 77(7), 3993-4003.