Mouse Anti-HK2 Monoclonal Antibody (VS7-0425-WR66)
CAT#: VS7-0425-WR66
This product is a mouse IgG antibody that binds to human HK2. The antibody can be used for WB, IHC-P, IF, FC, FC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/1912_G4_5_cr5c862f4c55d36_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61711/1101_E6_1_selected.jpg
Normal Tissue
Figure 3 Epididymis
High expression in epididymis
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/60634_A_1_6_val_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/60634_B_8_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/60634_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28587/60634_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000159399-HK2
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Specifications
- Immunogen
- Recombinant human HK2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HK2
- Species Reactivity
- Human
- Molecule Class
- Enzyme
- Applications
- WB, IHC-P, IF, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Cancer; Inflammatory diseases; Necrotizing enterocolitis (NEC) in newborns; Osteoarthritis
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Enzyme-linked Immunosorbent Assay: 1:10000
Flow Cytometry: 1:200 - 1:400
Immunohistochemistry: 1:200 - 1:1000
Western Blot: 1:500 - 1:2000
Target
- Alternative Names
- HKII; HXK2
- Gene ID
- 3099
- UniProt ID
- P52789
- Long Name
- Hexokinase 2
- Cellular Localization
- Cytoplasm; Membrane; Mitochondrion; Mitochondrion outer membrane
- Post Translation Modifications
- Acetylation
- Protein Refseq
- NP_000180.2
- Function
- Allosteric enzyme; Kinase; Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1615 | Rabbit Anti-HK2 Recombinant Antibody (clone DS1615AB) | WB, IHC-P, IF | Rabbit IgG |
| MOR-4588 | Rabbit Anti-HK2 Recombinant Antibody (clone TH101DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0618-WJ-F(E) | Mouse Anti-HK2 Recombinant Antibody; Fab Fragment (HPAB-0618-WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0618-WJ-S(P) | Mouse Anti-HK2 Recombinant Antibody; scFv Fragment (HPAB-0618-WJ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0420J | Mouse Anti-HK2 Recombinant Antibody (ZG-0420J) | WB | Mouse IgG |
| ZG-0421J | Mouse Anti-HK2 Recombinant Antibody (ZG-0421J) | WB | Mouse IgG |
| ZG-0475J | Mouse Anti-HK2 Recombinant Antibody (ZG-0475J) | WB | Mouse IgG |
| VS3-QX531 | Mouse Anti-HK2 Recombinant Antibody (clone 3B9-F8-F10) | WB | Mouse IgG1 |
| VS3-WK75 | Mouse Anti-HK2 Recombinant Antibody (clone F1-D3) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY249 | Rabbit Anti-NHP HK2 Recombinant Antibody (VS-1024-XY249) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1027 | Anti-HK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3162 | Anti-Human HK2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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