Anti-HLA-B Antibody Prodrug, Protease Activated (YTH 76.3)
CAT#: VS-1025-YC202
The Anti-HLA-B Antibody Prodrug, Protease Activated (YTH 76.3) is an antibody prodrug comprised of a recombinant rabbit antibody targeting human MHC-I B, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to HLA-B protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to HLA-B effectively. MHC-I B refers to HLA-B, a component of the MHC Class I complex required to present tumor antigens to T-cells. Its loss of expression is a common and potent mechanism of immune evasion used by cancer cells to escape recognition and killing by cytotoxic T-lymphocytes.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15418/35903_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15418/35903_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15418/35903_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15418/35903_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15418/35903_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15418/35903_A_9_8.jpg
Normal Tissue
Figure 7 Spleen
Cells in red pulp
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15418/35903_B_9_4.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A-431, EFO-21, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000234745-HLA-B
❮
❯
Specifications
- Host Animal
- Rabbit
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human MHC-I B
- Target
- HLA-B
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- YTH 76.3
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
- Alternative Names
- Major Histocompatibility Complex, Class I, B; HLAB; HLA Class I Histocompatibility Antigen, B-12 Alpha Chain
- UniProt ID
- Q96DW9
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Datasheet
MSDS
COA
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