What is the potential research application for this HMGB1 antibody?

This Mouse Anti-HMGB1 Recombinant Antibody Fab Fragment recognizes Human HMGB1. It has a potential use in studies related to Alzheimer's disease. HMGB1 is a nuclear DNA-binding protein involved in inflammation and cell differentiation, making this antibody a valuable tool for researching these cellular processes and their link to disease.

What type of antibody fragment is this product?

This product is a Mouse antibody Fab fragment (not a full-length IgG). It is engineered to recognize the High Mobility Group Box 1 protein. The Fab format is useful for specific applications where the Fc region is not desired. It is validated for use in ELISA and has>95% purity by SDS-PAGE.

Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-N0137-YC-F(E))

CAT#: HPAB-N0137-YC-F(E)

Provided is a recombinant Mouse antibody Fab fragment that recognizes HMGB1. It has a potential use in studies of Alzheimer's disease.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg

Subcellular Location Figure 3 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg

Subcellular Location Figure 4 IHC staining of human esophagus

Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg

Subcellular Location Figure 5 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg

Subcellular Location Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg

Normal Tissue Figure 8 Colon

Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg

RNA Expression Figure 12 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1

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Specifications

Host Species
Mouse

Type
Mouse Fab

Specificity
Human HMGB1

Species Reactivity
Human

Applications
ELISA

Related Disease
Alzheimer's disease

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
High Mobility Group Box 1; High-Mobility Group (Nonhistone Chromosomal) Protein 1; Sulfoglucuronyl Carbohydrate Binding Protein; High Mobility Group Protein 1; Amphoterin; HMG-1

Gene ID
3146

UniProt ID
P09429

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Breast cancer biomarkers at key points during disease progression

Useful Fab for HMGB1 Detection

I purchased this mouse antibody Fab fragment to study HMGB1 in the context of cellular inflammation processes. It worked very well in our ELISA setup. Using a Fab fragment was advantageous for our specific experimental design where we wanted to avoid Fc-mediated interactions, and the binding specificity was excellent.

High Quality Recombinant Reagent

The quality of this recombinant antibody is evident from the consistent results we get. We are investigating the role of High Mobility Group Box 1 protein in neurodegenerative research, and having a reliable tool like this is vital. It arrived on time, and the purity (>95%) confirmed by SDS-PAGE met our rigorous standards.

Q&As

What is the potential research application for this HMGB1 antibody?

A: This Mouse Anti-HMGB1 Recombinant Antibody Fab Fragment recognizes Human HMGB1. It has a potential use in studies related to Alzheimer's disease. HMGB1 is a nuclear DNA-binding protein involved in inflammation and cell differentiation, making this antibody a valuable tool for researching these cellular processes and their link to disease.
What type of antibody fragment is this product?

A: This product is a Mouse antibody Fab fragment (not a full-length IgG). It is engineered to recognize the High Mobility Group Box 1 protein. The Fab format is useful for specific applications where the Fc region is not desired. It is validated for use in ELISA and has>95% purity by SDS-PAGE.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-N0137-YC-F(E).pdf

MSDS

MSDS - HPAB-N0137-YC-F(E).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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