Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8)
CAT#: HPAB-0215-FY
This product is a recombinant antibody that recognizes Human HMGB1. The monoclonal antibody 3E 8 reacts with the epitope of HMGB1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
Subcellular Location
Figure 3 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
Subcellular Location
Figure 4 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
Subcellular Location
Figure 6 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
Normal Tissue
Figure 7 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
Normal Tissue
Figure 8 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HMGB1
- Species Reactivity
- Human
- Clone
- 3E8
- Applications
- Block, WB
- Related Disease
- Sepsis, systemic inflammatory response syndrome or acute lung injury, the autoimmune disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HMGB1 antibody has been reported in applications of Western Blot, Blocking. WB: Raw264.7 cells were stimulated with 1 µg/ml HMGB1 for 8 hours, with or without 50 µg/ml of the antibodies indicated.
Target
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Consistent Results in Diagnostic Development
This antibody offers exceptional consistency, crucial for diagnostic development. It provides reliable and reproducible results in detecting HMGB1, significantly improving diagnostic accuracy and efficiency.
Enhanced Accuracy in Cancer Research
The Mouse Anti-HMGB1 Recombinant Antibody from Creative Biolabs excels in cancer research. Its high sensitivity allows precise detection of HMGB1, enhancing the understanding of tumor biology and therapeutic targets.
Q&As
-
What are the primary applications of the Mouse Anti-HMGB1 Recombinant Antibody in scientific research?
A: The Mouse Anti-HMGB1 Recombinant Antibody is primarily used in various immunoassays, including ELISA, Western blotting, immunofluorescence, and immunohistochemistry. It is specifically designed to detect and quantify HMGB1 protein levels in different biological samples, aiding in the study of inflammation, cancer, and other diseases.
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How should samples be prepared for using the Mouse Anti-HMGB1 Recombinant Antibody in Western blotting?
A: For Western blotting, samples should be prepared by lysing cells or tissues in an appropriate buffer, followed by protein quantification. Denature the proteins by boiling them with SDS-PAGE sample buffer and then separate them using SDS-PAGE. Transfer the proteins to a membrane and incubate with the Mouse Anti-HMGB1 Recombinant Antibody.
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What concentration of Mouse Anti-HMGB1 Recombinant Antibody is recommended for immunohistochemistry?
A: For immunohistochemistry, it is recommended to use the Mouse Anti-HMGB1 Recombinant Antibody at a concentration of 1-10 µg/mL. Optimal concentrations should be determined experimentally based on the specific sample and detection system used.
-
How can non-specific binding be minimized when using the Mouse Anti-HMGB1 Recombinant Antibody?
A: To minimize non-specific binding, blocking steps are crucial. Use a blocking buffer containing BSA, non-fat dry milk, or normal serum for at least 1 hour before antibody incubation. Additionally, optimize the antibody dilution and incubation times.
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What types of detection systems are compatible with the Mouse Anti-HMGB1 Recombinant Antibody in ELISA assays?
A: The Mouse Anti-HMGB1 Recombinant Antibody is compatible with various detection systems, including colorimetric, chemiluminescent, and fluorescent detection methods. Choose a detection system based on the sensitivity and quantification requirements of the experiment.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 3E8"
- CAT
- Product Name
See other products for "HMGB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0315CL | Mouse Anti-HMGB1 Recombinant Antibody (TAB-0315CL) | IHC | Mouse IgG |
| TAB-0315CL-F(E) | Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-0315CL-F(E)) | ELISA, Inhib, FuncS, WB | Mouse Fab |
| TAB-0316CL-F(E) | Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-0316CL-F(E)) | ELISA, Inhib, FuncS, WB | Mouse Fab |
| TAB-0317CL-F(E) | Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-0317CL-F(E)) | ELISA, Inhib, FuncS, WB | Mouse Fab |
| TAB-0318CL-F(E) | Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-0318CL-F(E)) | ELISA, Inhib, FuncS, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-099CT | Human Anti-HMGB1 Recombinant Antibody (TAB-099CT) | IF, ELISA, IHC | Human IgG1, λ |
| TAB-099CT-S(P) | Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (TAB-099CT-S(P)) | ELISA, FC, Block | Human scFv |
| TAB-099CT-F(E) | Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (TAB-099CT-F(E)) | ELISA, FC, Block | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-101CT | Anti-Human HMG1 Recombinant Antibody | ELISA, BL | Human antibody |
| TAB-101CT-S(P) | Anti-Human HMG1 Recombinant Antibody scFv Fragment | ELISA, BL | Human antibody |
| TAB-101CT-F(E) | Anti-Human HMG1 Recombinant Antibody Fab Fragment | ELISA, BL | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0839CT | Recombinant Mouse anti-Human HMGB1 Monoclonal antibody (19N10B7) | ELISA, FC, ICC, IF, IHC-P, WB | |
| MRO-1938-CN | Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1938-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1939-CN | Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1939-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0101-WJ | Human Anti-HMGB1 Recombinant Antibody (HPAB-0101-WJ) | ELISA, WB | Humanized IgG1, λ |
| ZG-0740J | Rabbit Anti-HMGB1 Recombinant Antibody (clone 1A1) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1633 | Rabbit Anti-HMGB1 Recombinant Antibody (clone DS1633AB) | WB, IHC-P, FC | Rabbit IgG |
| MOR-0049-FY | Rabbit Anti-HMGB1 Recombinant Antibody (clone AFY0020) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2082 | PE-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer | FCM | |
| MHC-LC2083 | APC-B*07:02/Human HMGB1 (APKRPPSAF) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-FY-S(P) | Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); scFv Fragment | ELISA | Mouse scFv |
| HPAB-N0137-YC-S(P) | Mouse Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-N0137-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-0101-WJ-S(P) | Human Anti-HMGB1 Recombinant Antibody; scFv Fragment (HPAB-0101-WJ-S(P)) | ELISA, WB | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0215-FY-F(E) | Mouse Anti-HMGB1 Recombinant Antibody (clone 3E8); Fab Fragment | ELISA | Mouse Fab |
| HPAB-N0137-YC-F(E) | Mouse Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-N0137-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0101-WJ-F(E) | Human Anti-HMGB1 Recombinant Antibody; Fab Fragment (HPAB-0101-WJ-F(E)) | ELISA, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1654 | Recombinant Mouse Anti-HMGB1 Capture Antibody (clone 1B5) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC699 | AbPlus™ Anti-HMGB1 Magnetic Beads (VS-0724-YC699) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1033 | Anti-HMGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3204 | Anti-Mouse HMGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3206 | Anti-Human HMGB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3205 | Anti-Rat HMGB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC535 | CytoStream™ Rabbit Anti-HMGB1 Recombinant Antibody (VS13-YC535) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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