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    Rabbit Anti-HMGB1 Recombinant Antibody (clone AFY0020) (CAT#: MOR-0049-FY)

    This product is a recombinant Rabbit antibody that recognizes HMGB1. The monoclonal Rabbit antibody AFY0020 reacts with the epitope (c-terminal) of HMGB1.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line RH-30
    Figure 1 IF staining of human cell line RH-30
    (Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
    Figure 3 IHC staining of human bone marrow
    Figure 3 IHC staining of human bone marrow
    (Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
    Figure 4 IHC staining of human esophagus
    Figure 4 IHC staining of human esophagus
    (Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
    Figure 5 IF staining of human cell line U-2 OS
    Figure 5 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
    Figure 6 IF staining of human cell line U-251 MG
    Figure 6 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
    Normal Tissue
    Figure 7 Cerebral cortex
    Figure 7 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
    Figure 8 Colon
    Figure 8 Colon
    (Endocrine cells
    Staining: High
    Intensity: Strong
    Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Enterocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
    Figure 9 Liver
    Figure 9 Liver
    (Cholangiocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
    Figure 10 Kidney
    Figure 10 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1

    Specifications

    • Immunogen
    • C-terminal half of human High mobility group protein B1.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human HMGB1
    • Species Reactivity
    • Human, Mouse
    • Clone
    • AFY0020
    • Applications
    • ICC, IHC-P, WB
    • Conjugate
    • Unconjugated

    Product Property

    • Purity
    • >95% as determined by analysis by SDS-PAGE
    • Format
    • Lyophilized
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS, 5% Trehalose
    • Preservative
    • No preservatives
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody has been tested for use in Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot.

    Target

    • Alternative Names
    • High Mobility Group Box 1; High-Mobility Group (Nonhistone Chromosomal) Protein 1; Sulfoglucuronyl Carbohydrate Binding Protein; High Mobility Group Protein 1; Amphoterin; HMG-1

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "HMGB1"

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    CAT Product Name Application Type
    TAB-0315CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0315CL) IHC Mouse IgG
    TAB-0316CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0316CL) ELISA, Inhib, FuncS, WB Mouse IgG
    TAB-0317CL Mouse Anti-HMGB1 Recombinant Antibody (TAB-0317CL) ELISA, Inhib, FuncS, WB Mouse IgG
    TAB-100CT-S(P) Anti-Human HMG1 Recombinant Antibody scFv Fragment (TAB-100CT-S(P)) IF, FC
    TAB-100CT-F(E) Anti-Human HMG1 Recombinant Antibody Fab Fragment (TAB-100CT-F(E)) IF, FC

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