Rabbit Anti-HMGB1 Recombinant Antibody (clone CBACN-279)

Name: Rabbit Anti-HMGB1 Recombinant Antibody (clone CBACN-279)
SKU: MRO-0755-CN
Rating: 5 (1 reviews)

CAT#: MRO-0755-CN

This antibody is a rabbit monoclonal antibody that is capable of binding to HMGB1.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg

Subcellular Location Figure 3 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg

Subcellular Location Figure 4 IHC staining of human esophagus

Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg

Subcellular Location Figure 5 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg

Subcellular Location Figure 6 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg

Normal Tissue Figure 7 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg

Normal Tissue Figure 8 Colon

Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg

Normal Tissue Figure 10 Kidney

Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg

RNA Expression Figure 12 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1

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Specifications

Immunogen
Recombinant protein within Human HMGB1, aa 150 to the C-terminus

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human HMGB1

Species Reactivity
Human, Mouse, Rat

Clone
CBACN-279

Applications
WB, IF, IHC, FC

Conjugate
Unconjugated

Product Property

Purification
Protein A purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
1*TBS, pH 7.4, 1% BSA, 40% Glycerol

Preservative
0.05% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
High Mobility Group Box 1; High-Mobility Group (Nonhistone Chromosomal) Protein 1; Sulfoglucuronyl Carbohydrate Binding Protein; High Mobility Group Protein 1; Amphoterin; HMG-1;

Gene ID
3146

UniProt ID
P09429

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MRO-0755-CN.pdf

MSDS

MSDS - MRO-0755-CN.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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