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    CytoStream™ Rabbit Anti-HMGB1 Recombinant Antibody (VS13-YC535) (CAT#: VS13-YC535)

    The CytoStream™ Rabbit Anti-HMGB1 Recombinant Antibody is specific for human, mouse and rat HMGB1. HMGB1, a non-histone DNA-binding protein of the high mobility group superfamily, regulates transcription and is involved in DNA organization influencing cellular processes like inflammation and tumor migration, with pseudogenes identified. The anti-HMGB1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line RH-30
    Figure 1 IF staining of human cell line RH-30
    (Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
    Figure 3 IHC staining of human bone marrow
    Figure 3 IHC staining of human bone marrow
    (Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
    Figure 4 IHC staining of human esophagus
    Figure 4 IHC staining of human esophagus
    (Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
    Figure 5 IF staining of human cell line U-2 OS
    Figure 5 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
    Figure 6 IF staining of human cell line U-251 MG
    Figure 6 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
    Normal Tissue
    Figure 7 Cerebral cortex
    Figure 7 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
    Figure 8 Colon
    Figure 8 Colon
    (Endocrine cells
    Staining: High
    Intensity: Strong
    Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Enterocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
    Figure 9 Liver
    Figure 9 Liver
    (Cholangiocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
    Figure 10 Kidney
    Figure 10 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1

    Specifications

    • Immunogen
    • Recombinant human HMGB1, aa 151-200.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat HMGB1
    • Species Reactivity
    • Human, Mouse, Rat
    • Applications
    • WB, ICC, IF, IHC-P, FC
    • Conjugate
    • NONE

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A purified
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
    • Preservative
    • 0.05% Sodium azide
    • Storage
    • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Shipping with ice pack.

    Applications

    • Application Notes
    • Western blot (WB): 1/500-1/5000
      Immunocytochemistry (ICC): 1/100
      Immunofluorescence (IF): 1/100
      Immunohistochemistry-paraffin (IHC-P): 1/5000
      Flow cytometry (FC): 1/50

    Target

    • Alternative Names
    • High Mobility Group Box 1; High-Mobility Group (Nonhistone Chromosomal) Protein 1; Sulfoglucuronyl Carbohydrate Binding Protein; High Mobility Group Protein 1; Amphoterin; HMG-1;
    • Long Name
    • High Mobility Group Box 1
    • Cellular Localization
    • Secreted; Nucleus; Chromosome; Cytoplasm; Cell membrane

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "HMGB1"

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    CAT Product Name Application Type
    DrMAB-956 Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4) ELISA, WB Human IgG
    DrMAB-957 Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4), Biotin ELISA, WB Human IgG
    MRO-1938-CN Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1938-CN) WB, IF, IHC, FC Rabbit IgG
    MRO-1939-CN Rabbit Anti-HMGB1 Polyclonal Antibody (MRO-1939-CN) WB, IF, IHC, FC Rabbit IgG
    HPAB-0101-WJ Human Anti-HMGB1 Recombinant Antibody (HPAB-0101-WJ) ELISA, WB Humanized IgG1, λ

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